抗球虫药的人工抗原合成及免疫分析方法研究进展  

Research progress of artificial antigen synthesis and immunoassay for coccidiostats

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作  者:吴佳蓓 田恒旗 胡骁飞[2] 孙亚宁[2] 邢云瑞 王琳 王成宾 王耀[1] WU Jiabei;TIAN Hengqi;HU Xiaofei;SUN Yaning;XING Yunrui;WANG Lin;WANG Chengbin;WANG Yao(Food and Bioengineering College,Henan University of Science and Technology,Luoyang 471003,China;Key Laboratory of Animal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)

机构地区:[1]河南科技大学食品与生物工程学院,河南洛阳471003 [2]河南省农业科学院动物免疫学重点实验室,河南郑州450002

出  处:《食品与发酵工业》2024年第4期347-355,共9页Food and Fermentation Industries

基  金:国家自然科学基金(31702218);河南省科技攻关项目(232102320298);河南省青年人才托举工程(2020HYTP029);河南省研究生教育改革与质量提升工程项目(HNYJS2020 JD06)。

摘  要:抗球虫药在畜禽业应用广泛,但不合理使用会导致其在动物性食品中残留超标,对人类健康造成威胁。近年来,食品安全监督抽检中抗球虫药残留超标时有发生,因此,实现动物性食品中抗球虫药残留的高效快速检测,有利于兽药残留的有效监管。在众多残留检测方法中,免疫分析方法具有灵敏度高、特异性强,操作简单等优点,适用于大批量样品的现场快速检测。该文综述了抗球虫药人工抗原的合成方法,并对其免疫分析方法进行归纳总结,以期为开发更高效的抗球虫药残留分析方法提供参考。Coccidiostats have found wide applications in the livestock and poultry industry,but abuse of coccidiostats has caused the residue to exceed the limit in animal foods,thus posing a serious threat to human health.Considering the frequent occurrence of cases of this kind,it has been imperative to realize efficient detection of residue in animal foods,which can facilitate efficient monitoring of residue in veterinary medicine.Among a variety of residue detection approaches,immunoassay has been proven to be highly sensitive,highly specific,and easy to use.Because of these advantages,immunoassay has been widely used in a quick onsite test of samples in large batches.This paper reviewed the methods for the synthesis of coccidiostats artificial antigens and discussed immunoassay for coccidiostats residue detection,attempting to provide references for the development of more efficient coccidiostats residues.

关 键 词:抗球虫药 人工抗原 免疫分析 研究进展 

分 类 号:TS207.53[轻工技术与工程—食品科学]

 

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