核转录因子红系2相关因子2和p62在宫颈鳞状细胞癌和上皮内病变中的表达及诊断价值  

Expression and diagnostic value of Nrf2 and p62 in cervical squamous cell carcinoma and intraepithelial lesions

作　　者：郑桂华[1] 金晓霞 陶玉梅[2] 刘丹阳 朱兴华[1] 卫颖泽[1] ZHENG Guihua;JIN Xiaoxia;TAO Yumei;LIU Danyang;ZHU Xinghua;WEI Yingze(Department of Pathology,Nantong Tumor Hospital/Affiliated Tumor Hospital of Nantong University,Nantong,Jiangsu 226361,China;Department of Pathology,Nantong Maternal and Child Health Hospital,Nantong,Jiangsu 226007,China;Department of Pathology of the Obstetrics&Gynecology Hospital of Fudan University,Shanghai 200090,China)

机构地区：[1]南通市肿瘤医院/南通大学附属肿瘤医院病理科,江苏南通226361 [2]南通市妇幼保健院病理科,江苏南通226007 [3]复旦大学附属妇产科医院病理科,上海200090

出　　处：《安徽医药》2024年第3期491-495,I0005,共6页Anhui Medical and Pharmaceutical Journal

基　　金：国家自然科学基金项目(82103062);南通市卫健委面上项目(MA2021019,MB2020019)。

摘　　要：目的 探讨核转录因子红系2相关因子2(Nrf2)与选择性自噬接头蛋白p62/Sequestosome1 (SQSTM1)在诊断宫颈癌和上皮内病变中的价值,并且分析二者在不同宫颈病变中的相关性。方法 收集2008年1月至2021年12月南通市肿瘤医院120例宫颈鳞状细胞癌(SCC)、102例低级别鳞状上皮内病变(LSIL)和101例高级别鳞状上皮内病变(HSIL)病人及49例宫颈良性/反应性鳞状上皮病人的石蜡标本,免疫组织化学方法检测其中Nrf2和p62的表达,并分析二者在不同宫颈病变中的相关性及评估二者在诊断中的价值。结果 Nrf2和p62在LSIL、HSIL和SCC中表达明显高于良性/反应性宫颈鳞状上皮(均P<0.05),Nrf2和p62在HSIL和SCC中表达明显高于LSIL(均P<0.05),p62在SCC中表达明显高于HSIL(P<0.05),而Nrf2在HSIL中表达稍低于SCC(P<0.05)。Nrf2和p62在良性/反应性宫颈鳞状上皮、LSIL、HSIL和SCC中均具有正相关性,相关系数分别为0.63、0.58、0.69和0.38(均P<0.05)。ROC曲线结果显示,除Nrf2在诊断HSIL与SCC中差异无统计学意义外,Nrf2、p62以及联合Nrf2和p62在诊断良性/反应性鳞状上皮与LSIL、良性/反应性鳞状上皮与HSIL、良性/反应性鳞状上皮与SCC、LSIL与HSIL、LSIL与SCC、HSIL与SCC各个组别中均差异有统计学意义(均P<0.05)。结论 Nrf2和p62在良性/反应性鳞状上皮中不表达或低表达,LSIL中表达有所增高,HSIL和SCC中表达最高,二者在不同宫颈病变中均具有正相关性,而且单独使用Nrf2或p62就能够有效诊断不同的宫颈病变,二者联用诊断效果更佳。Objective To explore the value of nuclear factor-erythroid 2-related factor 2(Nrf2) and selective autophagy junction protein p62/Sequencosome1(SQSTM1) in the diagnosis of cervical cancer and intraepithelial lesions,and to analyze the correlation between Nrf2 and p62 in different cervical lesions.Methods The paraffin specimens of 120 cases with cervical squamous cell carcinoma(SCC),102 cases with low-grade squamous intraepithelial lesions(LSIL),101 cases with high-grade squamous intraepithelial lesions(HSIL),and 49 cases with benign/reactive squamous epithelium were collected in Nantong Tumor Hospital from January 2018 to December 2021.The expression levels of Nrf2 and p62 were evaluated through immunohistochemical staining.The correlation between Nrf2 and p62 and their value in diagnosis was also analyzed.Results The expression of Nrf2 and p62 in LSIL,HSIL and SCC was significantly higher than that in benign/reactive cervical squamous epithelium(P<0.05),the expression of Nrf2 and p62 in HSIL and SCC was also significantly higher than that in LSIL(P<0.05).The expression of p62 in SCC was significantly higher than that in HSIL(P<0.05),while the expression of Nrf2 in HSIL was slightly lower than that in SCC(P<0.05);Nrf2 and p62 were positively correlated in benign/reactive cervical squamous epithelium,LSIL,HSIL and SCC and their correlation coefficients were 0.63,0.58,0.69 and 0.38,respectively(P<0.05);ROC curve showed that Nrf2 had no statistical significance in the diagnosis of HSIL and SCC,while Nrf2,p62 and the combination of Nrf2 and p62 were statistically significant in the diagnosis of cervical benign/reactive squamous epithelium and LSIL,cervical benign/reactive squamous epithelium and HSIL,cervical benign/reactive squamous epithelium and SCC,LSIL and HSIL,LSIL and SCC,HSIL and SCC(P<0.05).Conclusions Nrf2 and p62 are not expressed or poorly expressed in benign/reactive squamous epithelium,the expression levels of Nrf2 and p62 are increased in LSIL,and highest in HSIL and SCC.Nrf2 is positively correlated wi

关 键 词：宫颈疾病 核转录因子红系2相关因子2 选择性自噬接头蛋白 良性/反应性 低级别 高级别 鳞状上皮内病变 鳞状细胞癌 

分 类 号：R737.33[医药卫生—肿瘤]