学龄前孤独症儿童额颞叶静息态功能连接特征近红外光谱成像研究  

A near-infrared spectroscopy study of brain resting state functional connectivity features of frontotemporal lobes in children with autism spectrum disorder

作　　者：黄浩宇[1] 王静 吴金庭 邹卓 魏贤召 程昱[1] 代蕊 王文娟[1] 陈颖娟 刘春明[1] 刘芸[1] HUANG Haoyu;WANG Jing;WU Jinting;ZOU Zhuo;WEI Xianzhao;CHENG Yu;DAI Rui;WANG Wenjuan;CHEN Yingjuan;LIU Chunming;LIU Yun(Department of rehabilitation,Kunming Children's Hospital(Children's Hospital Affiliated to Kunming Medical University),Kunming 650228,China)

机构地区：[1]昆明市儿童医院(昆明医科大学附属儿童医院)康复科,昆明650228

出　　处：《中国神经精神疾病杂志》2023年第12期734-739,共6页Chinese Journal of Nervous and Mental Diseases

基　　金：云南省科技厅昆医联合专项-面上项目(编号:202101AY070001-215);昆明市卫生健康委员会卫生科研课题(编号:2023-16-01-013,2023-16-01-001);昆明市卫生科技人才培养项目医学科技学科后备人才培养计划[编号:2022-SW(后备)-005,2021-SW(后备)-71]。

摘　　要：目的基于功能性近红外光谱成像技术(functional near-infrared spectroscopy,fNIRS)探索学龄前孤独症谱系障碍(autism spectrum disorder,ASD)儿童额颞叶的静息态脑功能连接(resting-state functional connectivity,rsFC)特征,寻找早期识别ASD可能的神经生物学标志。方法纳入63例学龄前ASD儿童和72名典型发育(typical development,TD)健康儿童,选取双侧背外侧前额叶皮质(dorsolateral prefrontal cortex,DLPFC)、双侧运动前皮质(premotor cortex,PMC)和双侧颞叶皮质(temporal lobe,TL)作为感兴趣区域(region of interest,ROI),使用fNIRS采集两组儿童静息态下氧合血红蛋白变化情况,比较功能连接强度并进行偏侧化分析。结果ASD组相较于TD组,rsFC强度降低有统计学意义(P<0.05),差异主要存在于左侧ROIs(0.21±0.11 vs.0.32±0.18)、右侧ROIs(0.16±0.16vs.0.30±0.14)、双侧DLPFC内(左0.20±0.14 vs.0.39±0.17;右0.15±0.13 vs.0.36±0.13)、双侧TL内(左0.15±0.14 vs.0.28±0.17;右0.14±0.15 vs.0.31±0.17),以及10组ROI-ROI之间(包括右DLPFC-左DLPFC、右DLPFC-右PMC、右DLPFC-左PMC、右DLPFC-右TL、右DLPFC-左TL、左DLPFC-右PMC、左DLPFC-左PMC、左DLPFC-右TL、左DLPFC-左TL、右TL-左TL)。ASD组与TD组DLPFC(t=2.002,P=0.047)和全脑(t=3.003,P=0.003)的偏侧化指数存在统计学差异,ASD组呈偏左侧化连接。结论学龄前ASD儿童额颞叶rsFC异常,主要表现为双侧DLPFC和双侧TL的短程功能连接降低、与DLPFC相关的长程功能连接降低以及偏左侧化连接等特征。Objective To explore the features of frontotemporal lobes'resting-state functional connectivity(rsFC)in preschool children with autism spectrum disorders(ASD)based on functional near-infrared spectroscopy(fNIRS)and to explore the possible neurological markers for early identification of ASD.Methods Sixty-three preschool ASD children and 72 typical development(TD)children were enrolled.Selected bilateral dorsolateral prefrontal cortex(DLPFC),bilateral premotor cortex(PMC),and bilateral temporal lobe(TL)cortex as the regions of interest(ROI).Changes of Oxyhemoglobin in the 6 ROIs in resting-state were measured by using functional near-infrared spectroscopy(fNIRS).Compared the frontotemporal rsFC strength and calculate the laterality index(LI)between two groups.Results Compared with the TD group,rsFC strength was significantly lower in the ASD group(P<0.05),and the differences existed mainly within the left ROIs(0.21±0.11 vs.0.32±0.18),right ROIs(0.16±0.16 vs.0.30±0.14),bilateral DLPFCs(0.20±0.14 vs.0.39±0.17;0.15±0.13 vs.0.36±0.13),bilateral TLs(0.15±0.14 vs.0.28±0.17;0.14±0.15 vs.0.31±0.17),and between the 10 groups of ROIs-ROIs(including right DLPFC-left DLPFC,right DLPFC-right PMC,right DLPFC-left PMC,right DLPFC-right TL,right DLPFC-left TL,left DLPFC-right PMC,left DLPFC-left PMC,left DLPFC-right TL,left DLPFC left TL,right TL-left TL).There were a significant differences in the rsFC's laterality index of DLPFC and whole-brain between the two groups(t=2.002,P=0.047;t=3.003,P=0.003),and the ASD group showed left-lateralized connectivity.Conclusion Frontotemporal lobe's resting-state functional connectivity is abnormal in preschool children with ASD,characterized by low short-range functional connectivity of bilateral DLPFCs and TLs,low long-range functional connectivity associated with DLPFCs,and left-lateralized connectivity.

关 键 词：孤独症谱系障碍 功能性近红外光谱成像技术 额颞叶 静息态功能连接 感兴趣区域 背外侧前额叶皮质 偏侧化指数 

分 类 号：R749.94[医药卫生—神经病学与精神病学]