GST family genes in jujube actively respond to phytoplasma infection  

作　　者：Qipeng Wang Liman Zhang Chaoling Xue Yao Zhang Xiangrui Meng Zhiguo Liu Mengjun Liu Jin Zhao 

机构地区：[1]College of Life Science,Hebei Agricultural University,Baoding,Hebei 071000,China [2]Hebei Key Laboratory of Plant Physiology and Molecular Pathology,Hebei Agricultural University,Baoding,Hebei 071000,China [3]Research Center of Chinese Jujube,Hebei Agricultural University,Baoding,Hebei 071000,China

出　　处：《Horticultural Plant Journal》2024年第1期77-90,共14页园艺学报（英文版）

基　　金：supported by grants from the National Key R&D Program Project Funding(Grant No.2018YFD1000607);the Foundation for 100 Innovative Talents of Hebei Province(Grant No.SLRC2019031);the National Natural Science Foundation of China(Grant No.31772285);the Hebei Province Innovation Foundation for Postgraduates(Grant No.CXZZBS2020097)。

摘　　要：Jujube witches’broom(JWB)caused by phytoplasma has a severely negative effect on multiple metabolisms in jujube.The GST gene family in plants participates in the regulation of a variety of biotic and abiotic stresses.This study aims to identify and reveal the changes in the jujube GST gene family in response to phytoplasma infection.Here,70 ZjGSTs were identified in the jujube genome and divided into 8 classes.Among them,the Tau-class,including 44 genes,was the largest.Phylogenetic analysis indicated that Tau-class genes were highly conserved among species,such as Arabidopsis,cotton,chickpea,and rice.Through chromosome location analysis,37.1%of genes were clustered,and 8 of 9 gene clusters were composed of Tau class members.Through RT-PCR,qRT-PCR and enzyme activity detection,the results showed that the expression of half(20/40)of the tested ZjGSTs was inhibited by phytoplasma infection in field and tissue culture conditions,and GST activity was also significantly reduced.In the resistant and susceptible varieties under phytoplasma infection,ZjGSTU49-ZjGSTU54 in the cluster IV showed opposite expression patterns,which may be due to functional divergence during evolution.Some upregulated genes(ZjGSTU45,ZjGSTU49,ZjGSTU59,and ZjGSTU70)might be involved in the process of jujube against JWB.The yeast two-hybrid results showed that all 6 Tauclass proteins tested could form homodimers or heterodimers.Overall,the comprehensive analysis of the jujube GST gene family revealed that ZjGSTs responded actively to phytoplasma infection.Furthermore,some screened genes(ZjGSTU24,ZjGSTU49-52,ZjGSTU70,and ZjDHAR10)will contribute to further functional studies of jujube-phytoplasma interactions.

关 键 词：Chinese jujube GST gene Family PHYTOPLASMA Gene cluster EXPRESSION Protein interaction 

分 类 号：S436.65[农业科学—农业昆虫与害虫防治]