Melatonin delays leaf senescence in pak choi(Brassica rapa subsp.chinensis)by regulating biosynthesis of the second messenger cGMP  

作　　者：Xuesong Liu Ronghui An Guofeng Li Shufen Luo Huali Hu Pengxia Li 

机构地区：[1]Institute of Agricultural Facilities and Equipment,Jiangsu Academy of Agricultural Sciences,Nanjing,Jiangsu 210014,China [2]School of Food and Biological Engineering,Jiangsu University,Zhenjiang,Jiangsu 212013,China [3]Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,Nanjing,Jiangsu 210014,China [4]Key Laboratory of Cold Chain Logistics Technology for Agro-Products,Ministry of Agriculture and Rural Affairs,Nanjing,Jiangsu 210014,China [5]Jinan Fruit Research Institute,All China Federation of Supply and Marketing Cooperatives,Jinan,Shandong 250220,China

出　　处：《Horticultural Plant Journal》2024年第1期145-155,共11页园艺学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant No.32001451);Jiangsu Agriculture Science and Technology Innovation Fund[Grant No.CX(20)1008]。

摘　　要：Melatonin(MT)is a low molecular weight compound with multiple biological functions in plants.It is known to delay leaf senescence in various species.However,no data are available on the MT signaling pathway in postharvest vegetables.This study demonstrates that MT increases cGMP concentration and the expression of the cGMP synthesis gene BcGC1 in pak choi.The c GMP inhibitor LY83583 destroys effect of MT delaying the leaf senescence.LY83583 also prevents MT treatment from reducing the expression of chlorophyll metabolism-related genes(BcNYC1,BcNOL,BcPPH1/2,BcSGR1/2,and BcPAO)and senescence genes(BcSAG12 and BcSAG21).It also inhibits MT from reducing the activity of the key chlorophyll catabolism enzymes Mg-dechelatase,pheophytinase,and pheide a oxygenase.Thus,the ability of MT to maintain high levels of chlorophyll metabolites is also destroyed.The Arabidopsis c GMP synthetic gene mutant atgc1 was used to confirm that delayed leaf senescence caused by MT is mediated,at least in part,by the second messenger c GMP.

关 键 词：Pak choi MELATONIN CGMP Chlorophyll degradation Senescence 

分 类 号：S634.3[农业科学—蔬菜学]