Characteristics of Salvia miltiorrhiza methylome and the regulatory mechanism of DNA methylation in tanshinone biosynthesis  

作　　者：Jiang Li Caili Li Yuxing Deng Hairong Wei Shanfa Lu 

机构地区：[1]Key Lab of Chinese Medicine Resources Conservation,State Administration of Traditional Chinese Medicine of the People’s Republic of China,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100193,China [2]Engineering Research Center of Chinese Medicine Resource,Ministry of Education,Beijing 100193,China [3]College of Forest Resources and Environmental Science,Michigan Technological University,Houghton,MI 49931,USA

出　　处：《Horticulture Research》2023年第7期265-279,共15页园艺研究（英文）

基　　金：This work was supported by the CAMS Innovation Fund for Medi-cal Sciences(CIFMS)(2021-I2M-2-001).

摘　　要：Salvia miltiorrhiza is a model medicinal plant with significant economic and medicinal value.Its roots produce a group of diterpenoid lipophilic bioactive components,termed tanshinones.Biosynthesis and regulation of tanshinones has attracted widespread interest.However,the methylome of S.miltiorrhiza has not been analysed and the regulatory mechanism of DNA methylation in tanshinone production is largely unknown.Here we report single-base resolution DNA methylomes from roots and leaves.Comparative analysis revealed differential methylation patterns for CG,CHG,and CHH contexts and the association between DNA methylation and the expression of genes and small RNAs.Lowly methylated genes always had higher expression levels and 24-nucleotide sRNAs could be key players in the RdDM pathway in S.miltiorrhiza.DNA methylation variation analysis showed that CHH methylation contributed mostly to the difference.Go enrichment analysis showed that diterpenoid biosynthetic process was significantly enriched for genes with downstream overlapping with hypoCHHDMR in July_root when comparing with those in March_root.Tanshinone biosynthesis-related enzyme genes,such as DXS2,CMK,IDI1,HMGR2,DXR,MDS,CYP76AH1,2OGD25,and CYP71D373,were less CHH methylated in gene promoters or downstream regions in roots collected in July than those collected in March.Consistently,gene expression was up-regulated in S.miltiorrhiza roots collected in July compared with March and the treatment of DNA methylation inhibitor 5-azacytidine significantly promoted tanshinone production.It suggests that DNA methylation plays a significant regulatory role in tanshinone biosynthesis in S.miltiorrhiza through changing the levels of CHH methylation in promoters or downstreams of key enzyme genes.

关 键 词：miltiorrhiza ROOTS MECHANISM 

分 类 号：S567.53[农业科学—中草药栽培]