下调PPAPDC1A的表达对结直肠癌细胞的增殖、侵袭和转移的影响  

Effect of down-regulation of PPAPDC1A expression on proliferation,invasion and metastasis of colorectal cancer cells

作　　者：郜培琼 千新来[2,3] 贺国洋[2,3] 原志庆[2] GAO Peiqiong;QIAN Xinlai;HE Guoyang;YUAN Zhiqing(Department of Pathology,the Second Affiliated Hospital of Xuzhou Medical University,Xuzhou 221000,China;Department of Pathophysiology,School of Basic Medical Sciences,Xinxiang Medical University,Xinxiang 453003,China;Department of Pathology,the Third Affiliated Hospital of Xinxiang Medical University,Xinxiang 453003,China)

机构地区：[1]徐州医科大学第二附属医院病理科,徐州221000 [2]新乡医学院基础医学院病理学教研室,新乡453003 [3]新乡医学院第三附属医院病理科,新乡453003

出　　处：《临床与实验病理学杂志》2024年第2期150-157,共8页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的探讨磷脂酸磷酸酶2域1A(PPAPDC1A)在结直肠癌组织和不同结直肠癌细胞系中的表达及意义。方法采用免疫组化EnVision法检测60例结直肠癌组织和配对癌旁正常组织中PPAPDC1A的表达。采用基因转染技术成功构建PPAPDC1A稳定过表达和沉默细胞株,通过CCK-8、Transwell、裸鼠皮下成瘤和裸鼠尾静脉注射等实验观察PPAPDC1A基因对不同结直肠癌细胞系的影响。结果与癌旁组织相比,PPAPDC1A在结直肠癌组织中表达上调,且PPAPDC1A的表达强度与细胞分化程度(P=0.011)呈负相关。成功构建PPAPDC1A稳定过表达和干扰细胞株。体内外实验结果显示,与Vector组相比,PPAPDC1A稳定过表达细胞株的生长速度(SW480-PPAPDC1A、RKO-PPAPDC1A组:0.38±0.03、0.25±0.01)、穿过小室的细胞数(SW480-PPAPDC1A、RKO-PPAPDC1A组:218.33±7.09、96.33±1.52)、克隆形成数量(SW480-PPAPDC1A、RKO-PPAPDC1A组:174.33±5.03、245.00±7.00)、体内肿瘤体积(4.16±0.91)、裸鼠肺转移灶数目(5.1±3.84)显著升高(P<0.05)。与NC组相比,PPAPDC1A沉默细胞株的生长速度(SW620-shPPAPDC1A、LOVO-shPPAPDC1A组:0.14±0.02、0.16±0.05)、穿过小室的细胞数(SW620-shPPAPDC1A、LOVO-shPPAPDC1A组:13.33±0.57、18.33±0.51)、克隆形成数量(SW620-shPPAPDC1A、LOVO-shPPAPDC1A组:28.33±1.52、8.67±0.57)、肿瘤体积(0.56±0.21)、裸鼠肺转移灶数目(1.2±1.03)显著降低(P<0.05)。结论下调PPAPDC1A表达可以抑制结直肠癌细胞的增殖、侵袭、迁移和转移能力。Purpose To investigate the expression and relationship of phosphatidic acid phosphatase 2 domain 1A(PPAPDC1A),also known as phospholipid phosphatase 4(PLPP4),in colorectal cancer(CRC)tissues and different colorectal cancer cells.Methods Immunohistochemical EnVision method was applied to detect the expression of PPAPDC1A in 60 CRC tissues and paired paracancerous tissues.Stable overexpression and silencing cell lines of PPAPDC1A were successfully constructed by gene transfection,and the effects of this gene on different colorectal cancer cell lines were investigated by CCK-8,Transwell,subcutaneous tumor formation in nude mice and tail vein injection in nude mice.Results PPAPDC1A expression was upregulated in CRC tissues compared with paracancerous tissues,and the intensity of PPAPDC1A expression was negatively correlated with cell differentiation(P=0.011).PPAPDC1A stable overexpression and interference cell lines were successfully constructed.The results of in vitro and in vivo experiments showed that the growth rate(SW480-PPAPDC1A,RKO-PPAPDC1A groups:0.38±0.03,0.25±0.01),the number of cells crossing the compartment(SW480-PPAPDC1A,RKO-PPAPDC1A groups:218.33±7.09,96.33±1.52),the number of clone formation(SW480-PPAPDC1A,RKO-PPAPDC1A groups:174.33±5.03,245.00±7.00),the in vivo tumor volume(4.16±0.91),and the number of lung metastasis in nude mice(5.1±3.84)were significantly higher in the PPAPDC1A stably overexpressing cell lines compared with the Vector group(P<0.05).However,the growth rate(SW620-shPPAPDC1A,LOVO-shPPAPDC1A groups:0.14±0.02,0.16±0.05),number of cells crossing the chambers(SW620-shPPAPDC1A,LOVO-shPPAPDC1A groups:13.33±0.57,18.33±0.51),number of clone formation(SW620-shPPAPDC1A,LOVO-shPPAPDC1A groups:28.33±1.52,8.67±0.57),tumor volume(0.56±0.21),and number of lung metastasis in nude mice(1.2±1.03)were significantly lower(P<0.05)in the PPAPDC1A-silenced cell line compared with the NC group.Conclusion Down-regulation of PPAPDC1A expression inhibits the proliferation,invasion,migration and

关 键 词：结直肠肿瘤 转移 PPAPDC1A 增殖 侵袭 

分 类 号：R735.3[医药卫生—肿瘤]