鲸偶蹄目RNase11和RNase12的分子进化研究  

Molecular evolution of the RNase11 and RNase12 in Cetartiodactyla

作　　者：郎大田 李高银 张毅 刘松菊 LANG Datian;LI Gaoyin;ZHANG Yi;LIU Songju(Agronomy and Life Science Department,Zhaotong University,Zhaotong 657000,China;Animal Health Supervision Institute of Zhaoyang District,Zhaotong City,Yunnan Province,Zhaotong 657000,China;Animal Health Supervision Institute of Zhaotong City,Yunnan Province,Zhaotong 657000,China)

机构地区：[1]昭通学院农学与生命科学学院,云南昭通657000 [2]云南省昭通市昭阳区动物卫生监督所,云南昭通657000 [3]云南省昭通市动物卫生监督所,云南昭通657000

出　　处：《安徽大学学报（自然科学版）》2024年第1期72-84,共13页Journal of Anhui University(Natural Science Edition)

基　　金：云南省地方本科高校基础研究联合专项资金面上项目(202101BA070001-068);云南省科技特派员项目(202104BK090932);云南省中青年学术和技术带头人后备人才项目。

摘　　要：脊椎动物特有的RNaseA超家族因频繁发生基因重复和功能分化,一直被认为是分子进化研究的经典模型.过去研究主要聚焦于RNaseA超家族中与食性相关的典型成员RNase1,而非典型成员的研究非常有限.笔者以鲸偶蹄目4个亚目21科92个物种为研究对象,基于系统发育树、等电点、选择压力分析、蛋白质结构等方法深入开展RNaseA超家族非典型成员RNase11和RNase12的分子进化研究,共获得69条RNase11和88条RNase12序列,其中RNase11在水生的河马形亚目27个物种中发生假基因化或基因丢失.RNase11和RNase12的平均等电点分别为8.58,8.25,揭示RNase11和RNase12在鲸偶蹄目中可能拥有与生殖相关的抗菌功能.系统发育树显示鲸偶蹄目的胼足亚目和猪形亚目形成姐妹群最先发生分歧,其次是河马形亚目,最后是反刍亚目.此外,选择压力分析揭示RNase11和RNase12在进化过程中分别有7个和9个位点受到正选择,揭示RNase11和RNase12生物学功能可能发生了改变.总之,通过系统开展鲸偶蹄目RNase11和RNase12的分子进化研究,为进一步认识RNaseA超家族非典型成员和鲸偶蹄目的进化奠定了一定的基础,丰富了RNaseA超家族分子进化研究的多样性.The ribonuclease(RNase)A superfamily belongs to vertebrate-specific gene family,that is one of the best models for molecular evolutionary studies for it displays the frequent occurrence of gene duplications and functional diversification in multiple mammalian lineages.Previous studies mainly focused on RNase1 that is associated with dietary adaptation,whereas research on the non-typical members of the RNaseA superfamily has been limited.In the study,RNase11 and RNase12 from 92 species of 21 families in 4 suborders were analyzed based on phylogenetic tree,isoelectric point,selection pressure and protein structure.A total of 69 RNase11 and 88 RNase12 sequences were identified,the RNase11 was lost or pseudogenization appeared in the 27 Cetancodonta species.The average isoelectric points were 8.58 and 8.25 in the RNase11 and RNase12 respectively,suggesting they may possess the antibacterial function with reproduction.Phylogenetic tree demonstrated that Tylopoda and Suoidea formed sister group as the?rst lineage to diverge,subsequently is the Cetancodonta,and finally the Ruminantia.Besides,seven and nine positive sites were identified in RNase11 and RNase12 respectively,suggesting the function may be changed in RNase11 and RNase12 during the evolution.In sum,this study provided a complex and intriguing evolutionary history for the RNase11 and RNase12 in Cetartiodactyla,laying a solid foundation for further understanding the non-typical members of the RNaseA superfamily,and demonstrated the diversity about the RNaseA superfamily.

关 键 词：鲸偶蹄目 RNase11 RNase12 正选择 分子进化 

分 类 号：Q111[生物学—普通生物学]