经皮穴位电刺激对去势后大鼠血清骨代谢平衡的影响  

Effect of transcutaneous electrical acupoint stimulation on the balance of serum bone metabolic indexes in ovariectomized rats

作　　者：郑小花 徐颖[1] 林超琴[1] 胡德婷 郑启斌 ZHENG Xiaohua;XU Ying;LIN Chaoqin;HU Deting;ZHENG Qibin(Fujian Maternity and Child Health Hospital,College of Clinical Medicine for Obstetrics&Gynecology and Pediatrics,Fujian Medical University,Fuzhou 350001;Department of Obstetrics and Gynecology,The CangShan District of the 900th Hospital,Fuzhou 350025;Department of Neurosurgery,the First Affiliated Hospital of Fujian Medical University,Fuzhou 350001;Department of Neurosurgery,National Regional Medical Center,Binhai Campus of the First Affiliated Hospital of Fujian Medical University,Fuzhou 350212,China)

机构地区：[1]福建省妇幼保健院福建医科大学妇儿临床医学院,福建福州350001 [2]中国人民解放军联勤保障部队第九〇〇医院仓山院区妇产科,福建福州350025 [3]福建医科大学附属第一医院神经外科,福建福州350001 [4]福建医科大学附属第一医院滨海院区国家区域医疗中心神经外科,福建福州350212

出　　处：《中国骨质疏松杂志》2024年第1期1-5,共5页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金(82171472);福建省自然科学基金(2020J01990,2020J011145);福建省科技创新联合资金项目(2020Y9136)。

摘　　要：目的观察经皮穴位电刺激(transcutaneous electrical acupoint stimulation,TEAS)对去势后SD大鼠血清骨代谢指标及骨密度(bone mineral density,BMD)的影响。方法大鼠随机分为Sham组(假手术组)、0VX组(模型对照组)、2 Hz组(0VX+TEAS 2 Hz组)、50 Hz组(0VX+TEAS 50 Hz组),每组8只,3个月后分别检测血清骨代谢指标骨特异性碱性磷酸酶(BALP)、I型原胶原C端前肽(PICP)、I型原胶原N端前肽(PINP)、I型胶原羧基端肽(ICTP)、吡啶啉(Pyr)、脱氧吡啶啉(D-Pyr)及BMD。结果0VX组较Sham组血清BALP、PICP、PINP、ICTP、Pyr、D-Pyr均显著增高(P<0.05);与OVX组比较,2 Hz组血清BALP、PINP、PICP、ICTP、Pyr、D-Pyr均显著降低,腰椎、右股骨BMD增加(P<0.05),50 Hz组血清BALP、PINP、PICP及BMD无明显变化,ICTP、Pyr、D-Pyr亦均显著降低(P<0.05);与50 Hz组比较,2 Hz组腰椎BMD增高(P<0.05)。结论经皮穴位电刺激可能通过调控骨吸收和骨形成指标,参与抗骨质疏松作用,且2 Hz电刺激频率可能较50 Hz更适用于临床骨质疏松症的防治。Objective To observe the effects of transcutaneous electrical acupoint stimulation(TEAS)on serum bone metabolic indexes and bone mineral density(BMD)in ovariectomized SD rats.Methods Thirty-two rats were randomly divided into Sham group(sham operation group),OVX group(model control group),2 Hz group(OVX+TEAS 2 Hz group),and 50 Hz group(OVX+TEAS 50 Hz group),with 8 rats in each group.Serum bone metabolic indexes,including bone specific alkaline phosphatase(BALP),type I procollagen C-terminal propeptide(PICP),type I procollagen N-terminal propeptide(PINP),type I collagen carboxyl-terminal peptide(ICTP),pyridinoline(Pyr),deoxypyridinoline(D-Pyr),and BMD were detected 3 months later.Results Compared to those in Sham group,serum BALP,PINP,PICP,ICTP,Pyr,and D-Pyr in OVX group increased significantly(P<0.05).Compared to those in OVX group,serum BALP,PINP,PICP,ICTP,Pyr,and D-Pyr in 2 Hz group decreased significantly.BMD in lumbar spine and right femur increased(P<0.05).BALP,PINP,PICP,and BMD in 50 Hz group had no significant changes.ICTP,Pyr,and D-Pyr also decreased significantly(P<0.05).Compared to that in the 50 Hz group,the lumbar BMD in the 2 Hz group was higher(P<0.05).Conclusion TEAS play a role in anti-osteoporosis,probably by regulating bone resorption and bone formation indexes.The electrical stimulation with frequency of 2 Hz may be more suitable for clinical prevention and treatment of osteoporosis than with 50 Hz.

关 键 词：经皮电刺激 绝经后骨质疏松症 骨代谢 骨密度 大鼠 

分 类 号：R224[医药卫生—中医基础理论] R-332[医药卫生—中医学]