脊髓Mas相关基因受体C与小鼠骨癌痛病理生理机制的关系  

Relationship between spinal Mas-related gene receptor C and pathophysiological mechanism of bone cancer pain in mice

作　　者：王爱桃 姚尚龙[2] 郭冬梅 姜晖 程宏宇 乌云格日勒 白艳艳[1] Wang Aitao;Yao Shanglong;Guo Dongmei;Jiang Hui;Cheng Hongyu;Wu Yungerile;Bai Yanyan(Department of Anesthesiology,the First Hospital of Hohhot,Hohhot 010030,China;Department of Anesthesiology,Union Hospital,Tongji Medical College of Huazhong University of Science and Technology,Wuhan 430022,China;Department of Anesthesiology,Inner Mongolia Autonomous Region People′s Hospital,Hohhot 010017,China)

机构地区：[1]呼和浩特市第一医院麻醉科,呼和浩特010030 [2]华中科技大学同济医学院附属协和医院麻醉科,武汉430022 [3]内蒙古自治区人民医院麻醉科,呼和浩特010017

出　　处：《中华麻醉学杂志》2023年第12期1470-1472,共3页Chinese Journal of Anesthesiology

基　　金：内蒙古医科大学联合项目(YKD2021LH049);内蒙古自治区卫生健康科技计划项目(202201066)。

摘　　要：目的探讨脊髓Mas相关基因受体C(MrgC)与小鼠骨癌痛病理生理机制的关系。方法SPF级雄性C3H/HeNCrlVr小鼠40只,5~7周龄,体质量20~25 g,采用随机数字表法分为4组(n=10):假手术组(S组)、骨癌痛组(P组)、骨癌痛+MrgC激动剂组(P-agonist组)和骨癌痛+MrgC抗体组(P-Ab组)。骨癌痛模型的制备:P组、P-agonist组和P-Ab组小鼠胫骨上端注射小鼠纤维肉瘤细胞(NCTC2472),S组胫骨上端注射等容量Dhank′s平衡盐溶液(NCTC2472溶媒);14 d后,S组和P组小鼠鞘内注射人工脑脊液,P-agonist组和P-Ab组小鼠鞘内注射MrgC激动剂、MrgC抗体。于骨癌痛造模前(T_(0))、骨癌痛造模开始后7 d(T_(1))、14 d(鞘内注射前,T_(2))、鞘内注射后4 h(T_(3))、8 h(T_(4))和12 h(T_(5))时测定机械缩足反应阈(MWT)。结果与S组比较,T_(0)时其余各组MWT差异无统计学意义(P>0.05),T_(1)-T_(5)时MWT降低(P<0.05);与P组比较,T_(3)-T_(5)时P-agonist组MWT升高,T_(3)-T_(5)时P-Ab组MWT降低(P<0.05)。结论脊髓MrgC在小鼠骨癌痛病理生理机制中,发挥一定程度内源性保护性作用。Objective To investigate the relationship between spinal Mas-related gene receptor C(MrgC)and pathophysiological mechanism of bone cancer pain in mice.Methods Forty male C3H/HeNCrlVr mice,aged 5-7 weeks,weighing 20-25 g,were selected and divided into 4 groups(n=10 each)using a random number table method:sham operation group(group S),bone cancer pain group(group P),bone cancer pain+MrgC agonist group(group P-agonist)and bone cancer pain+Mrg C antagonist group(group P-Ab).Preparation of the bone cancer pain model:mouse fibrosarcoma cells(NCTC2472)were injected into the upper tibia of mice in P,P-agonist and P-Ab groups,and the equal volume of D-Hanks balanced salt solution was given instead in S group.Fourteen days later cerebrospinal fluid was intrathecally injected in S and P groups,and MrgC agonist and MrgC antibody were intrathecally injected in P-agonist and P-Ab groups.The mechanical paw withdrawal threshold(MWT)to von Frey stimuli was measured before developing the model(T_(0)),at 7 days after developing the model(T_(1)),at 14 days after developing the model(before intrathecal injection,T_(2)),and at 4,8 and 12 h after intrathecal injection(T_(3-5)).Results Compared with group S,no significant change was found in the MWT at T_(0)(P>0.05),and the MWT was significantly decreased at T_(1)-T_(5) in the other groups(P<0.05).Compared with group P,the MWT was significantly increased at T_(3)-T_(5) in group P-agonist,and the MWT was significantly decreased at T_(3)-T_(5) in group P-Ab(P<0.05).Conclusions Spinal MrgC plays an endogenous protective role in the pathophysiological mechanism of bone cancer pain to a certain extent in mice.

关 键 词：受体 G蛋白偶联 骨肿瘤 癌痛 脊髓 

分 类 号：R738.1[医药卫生—肿瘤]