鳖甲煎丸对肝癌大鼠黏着斑激酶/雷帕霉素靶蛋白通路的影响  

Effects of Biejia Jianwan on liver histopathological features and FAK/mTORC1 pathway in rats with hepatocellular carcinoma

作　　者：李杳瑶[1] 刘华[1] 孙铜林 伍静 孟小莎 伍梦思 苏联军[2] LI Yaoyao;LIU Hua;SUN Tonglin;WU Jing;MENG Xiaosha;WU Mengsi;SU Lianjun(The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]湖南中医药大学,湖南长沙410208

出　　处：《陕西中医》2024年第3期308-312,共5页Shaanxi Journal of Traditional Chinese Medicine

基　　金：湖南省自然科学基金资助项目(2021JJ30523);湖南省中医肿瘤临床医学研究中心项目(2021SK4023)。

摘　　要：目的:探讨鳖甲煎丸对肝癌模型大鼠肝组织病理特征及FAK/mTORC1通路的影响。方法:HepG2(1×10^(7))细胞注射于大鼠右腋皮下,建立肝癌模型,分为模型组、5-氟尿嘧啶组、鳖甲煎丸低剂量组、鳖甲煎丸高剂量组,另设正常组。各药物组给予相应药物灌胃,持续给药4周,正常组、模型组给予等体积的0.9%氯化钠溶液。实验结束后,测定肝癌组织重量、肝癌组织体积、肝癌组织抑瘤率、肝癌FAK、mTORC1水平、肝癌VEGFR-2、VEGF、IL-4、IL-8、TNF-α蛋白水平。结果:与正常组比较,模型组肝癌组织重量、肝癌组织体积、肝癌组织抑瘤率、VEGFR-2、VEGF、IL-4、IL-8、TNF-α蛋白、肝癌FAK、mTORC1 mRNA和蛋白表达升高(均P<0.05)。与模型组比较,5-氟尿嘧啶组、鳖甲煎丸低剂量组、鳖甲煎丸高剂量组肝癌组织重量、肝癌组织体积、肝癌组织抑瘤率、VEGFR-2、VEGF、IL-4、IL-8、TNF-α蛋白、肝癌FAK、mTORC1 mRNA和蛋白表达降低(均P<0.05)。鳖甲煎丸高剂量组肝癌组织重量、肝癌组织体积、肝癌组织抑瘤率、VEGFR-2、VEGF、IL-4、IL-8、TNF-α蛋白、肝癌FAK、mTORC1 mRNA和蛋白表达低于鳖甲煎丸低剂量组(均P<0.05)。结论:鳖甲煎丸对大鼠肝癌具有明显抑制作用,能减轻大鼠肝癌所致的病理损伤,其机制可能与鳖甲煎丸抑制肝癌FAK、mTORC1表达,诱导肝癌细胞凋亡,降低炎症反应有关。Objective:To explore effect of Biejia Jianwan on liver histopathological features and FAK/mTORC1 pathway in rats with hepatocellulaRcarcinoma.Methods:HepG2(1×10^(7))cells were injected subcutaneously into the right armpit of rats to establish livercanceRmodel,which were divided into model control group,5-fluorouracil group,Biejia Jianwan low-dose group and Biejia Jianwan high-dose group,and a normal control group.Each drug group were given corresponding drugs administered intragastrically foR4 weeks,and the normal group and the model control groups were given equal volumes of normal saline.AfteRthe test,livercanceRtissue weight,livercanceRtissue volume,livercanceRtissue tumoRinhibition rate,livercanceRFAK and mTORC1 levels,and livercanceRVEGFR-2,VEGF,IL-4,IL-8,and TNF-αprotein levels were measured.Results:Compared with the normal control group,livercanceRtissue weight,livercanceRtissue volume,tumoRinhibition rate,VEGFR-2,VEGF,IL-4,IL-8,TNF-αprotein,livercanceRFAK,mTORC1 mRNA and protein expression in the model group were increased(all P<0.05).Compared with the model group,livercanceRtissue weight,livercanceRtissue volume,livercanceRtumoRinhibition rate,VEGFR-2,VEGF,IL-4,IL-8,TNF-αprotein,livercanceRFAK,mTORC1 mRNA and protein expression decreased in the 5-fluorouracil group,Biejia Jianwan low-dose group and Biejia Jianwan high-dose group(all P<0.05).LiveRcanceRtissue weight,livercanceRtissue volume,tumoRinhibition rate,VEGFR-2,VEGF,IL-4,IL-8,TNF-αprotein,livercanceRFAK,mTORC1 mRNA and protein expression in Biejia Jianwan high-dose group were lowerthan those in the Biejia Jianwan low-dose group(all P<0.05).Conclusion:Biejia Jianwan has obvious inhibitory effect on livercanceRin rats,and can reduce pathological damage caused by livercanceRin rats.Biejia Jianwan can inhibit expression of FAK and mTORC1 in livercanceRcells,induce apoptosis and reduce inflammatory response in livercanceRcells.

关 键 词：肝癌 鳖甲煎丸 斑激酶 雷帕霉素靶蛋白 肝癌组织重量 抑瘤率 

分 类 号：R735.7[医药卫生—肿瘤]