基于低共熔溶剂的分散液液微萃取-高效液相色谱法测定环境水样和饮料中的柯依定、金胺O和罗丹明B  

Determination of chrysoidin,auramine O and rhodamine B in environmental water and beverage by deep eutectic solvents based dispersive liquid-liquid microextraction combined with high performance liquid chromatography

作　　者：于洋 曹天荟 罗安梦 高树林 葛丹丹 YU Yang;CAO Tianhui;LUO Anmeng;GAO Shulin;GE Dandan(School of Chemistry and Chemical Engineering,Kunming University,Kunming 650214,China)

机构地区：[1]昆明学院化学化工学院,昆明650214

出　　处：《分析试验室》2024年第1期104-110,共7页Chinese Journal of Analysis Laboratory

基　　金：云南省地方本科高校基础研究联合专项资金项目面上项目(ZX20220116)资助。

摘　　要：建立了基于低共熔溶剂的涡旋辅助分散液液微萃取-高效液相色谱联用检测柯衣定、金胺O和罗丹明B的方法。以香芹酚为氢键给体,薄荷醇、正癸酸或正辛酸为氢键受体,制备疏水性低共熔溶剂(DESs)并作为萃取溶剂。采用红外光谱(FT-IR)和量子化学计算研究DESs的形成机理。对DESs组成、萃取溶剂体积、萃取时间、溶液pH和盐浓度等实验条件进行了优化。在最优化条件下,柯衣定、金胺O和罗丹明B在5~1000 ng/mL范围内线性关系良好,相关系数均大于0.9950,检出限和定量限分别为1.0~1.5 ng/mL和3.5~4.7 ng/mL。模拟计算研究表明,DESs萃取3种合成色素的主要作用力是氢键和静电相互作用力。将所建立的方法用于检测环境水样和饮料样品中的金胺O、罗丹明B和柯衣定,加标回收率为85.7%~106.8%,相对标准偏差为0.2%~7.7%。A vortex-assisted liquid-liquid microextraction method combined with high performance liquid chromatography-diode array detector was established for the determination of chrysoidin,auramine O and rhodamine B in environmental water and beverage.The water-immiscible deep eutectic solvents(DESs) were prepared using carvacrol as hydrogen-bonding donor and menthol,n-decanoic acid or n-octanoic acid as hydrogen-bonding acceptor at different molar ratios,and the DESs were used as extraction solvents.The formation mechanism of DESs was investigated using Fourier transform infrared spectroscopy(FT-IR) and quantum chemical calculations.Several parameters influencing the extraction efficiencies of synthetic dyes including types of DESs,volume of extraction solvent,extraction time,pH of solution and salt concentration were optimized.Under the optimum conditions,synthetic dyes can be determined in the range of 5.0-1000 ng/mL with the coefficient higher than 0.9950.Limits of detection of the method ranged from 1 to 1.5 ng/mL and limits of quantification were in a range of 3.5-4.7 ng/mL.The possible interactions of DESs with synthetic dyes were investigated by computational modeling.The results revealed that hydrogen bonds and electrostatic interactions were the main driving forces for the extraction of synthetic dyes.The proposed method was applied to determine the synthetic dyes in environmental water and beverage with the recoveries in the range of 85.7%-106.8% and the relative standard deviations(RSDs) between 0.2%-7.7%.

关 键 词：疏水性低共熔溶剂 模拟计算 合成色素 分散液-液微萃取 高效液相色谱法 

分 类 号：O661.1[理学—分析化学]