甲基化PAX1在宫颈癌筛查和预后评估中的临床应用  

Clinical application of methylated PAX1 in cervical cancer screening and prognosis assessment

作　　者：刘静[1] LIU Jing(Oncology and Gynecology Department of The First Affiliated Hospital of Bengbu Medical College,Bengbu 233000,Anhui,China)

机构地区：[1]蚌埠医学院第一附属医院肿瘤妇科,安徽蚌埠233000

出　　处：《吉林医学》2024年第3期525-528,共4页Jilin Medical Journal

基　　金：蚌埠医学院自然科学重点项目[项目编号:2020byzd133]。

摘　　要：目的:探究甲基化PAX1在宫颈癌筛查和预后评估中的临床应用。方法:选取2021年1月~2021年12月在蚌埠医学院第一附属医院接受治疗的150例收集宫颈脱落细胞患者的宫颈脱落细胞,研究采用了多种检测技术,包括PAX1基因、甲基化、薄层细胞学、高危型人乳头瘤病毒(HR-HPV)和HPV16/18检测,以阳性患者进行阴道镜下活检病理诊断为金标准,比较宫颈脱落细胞中不同检测方法学。结果:PAX1基因甲基化灵敏度、特异度分别为81.3%和96.6%,四种检测比较中PAX1具有最高特异度(96.63%),细胞学与HR-HPV检测特异度在17%~70%。细胞学(ASCUS+)的灵敏度为94.7%,明显高于PAX1基因甲基化检测,差异有统计学意义(P<0.05),ASCUS+的特异度为18.0%显著低于PAX1基因甲基化检测,差异有统计学意义(P<0.001)。结论:将细胞学和PAX1基因甲基化技术相结合,能够更好地预防宫颈癌,并且能够减轻年轻患者的焦虑。Objective To explore the clinical application of methylated PAX1 in cervical cancer screening and prognosis assessment.Method The study involved 150 women treated between January and December 2021,and collected cervical exfoliated cells from patients with exfoliated cervical cells.The study used a variety of testing techniques,including PAX1 gene,methylation,thin layer cytology,high-risk HPV virus and HPV16/18 testing.Colposcopic biopsy of positive patients was used as the gold standard to compare different detection methods in cervical exfoliated cells.Results The methylation sensitivity and specificity of PAX1 gene were 81.3%and 96.6%,respectively.Among the four kinds of detection,PAX1 had the highest specificity of 96.63%in cytology and 17%~70%in hrHPV.The sensitivity of ASCUS+was 94.7%,which was significantly higher than that of PAX1 gene methylation detection(P<0.05),but the specificity of ASCUS+was 18.0%,which was significantly lower than that of PAX1 gene methylation detection(P<0.001).Conclusion The combination of cytology and PAX1 gene methylation technology can better prevent cervical cancer and reduce anxiety in young patients.

关 键 词：甲基化PAX1 宫颈癌筛查 预后评估 临床应用 

分 类 号：R737.33[医药卫生—肿瘤]