安罗替尼通过NF-κB信号通路对脑胶质瘤细胞增殖、凋亡的影响  

Effects of anlotinib regulating NF-κB signaling pathway on proliferation and apoptosis of glioma cells

作　　者：柳新 谢云鹏[2] 张圣林[1] 李青山[1] 董怡 LIU Xin;XIE Yunpeng;ZHANG Shenglin;LI Qingshan;DONG Yi(Department of Oncology,The Affiliated Hospital of Chengde Medical College,Chengde 067000,China;不详)

机构地区：[1]承德医学院附属医院肿瘤科,河北承德067000 [2]承德医学院附属医院神经外科

出　　处：《山东医药》2024年第7期48-51,共4页Shandong Medical Journal

基　　金：河北省卫生健康委员会医学科学研究课题(2023136)。

摘　　要：目的探讨安罗替尼通过核因子κB(NF-κB)信号通路对人脑胶质瘤细胞(T98G细胞)增殖、凋亡的影响。方法体外培养T98G细胞,并将其随机分为对照组、5μmol/L安罗替尼组、10μmol/L安罗替尼组、20μmol/L安罗替尼组、阳性药物组、抑制剂组。对照组不予干预,5μmol/L安罗替尼组、10μmol/L安罗替尼组、20μmol/L安罗替尼组分别以5、10、20μmol/L安罗替尼进行干预,阳性药物组以50 mg/L的5-氟尿嘧啶进行干预,抑制剂组加入10μmol/L安罗替尼+5μmol/L NF-κB通路抑制剂BAY 11-7082进行干预。用CCK-8法检测细胞活力,用5-乙炔基-2'脱氧尿嘧啶核苷法测算细胞增殖率,用Hoechst33258染色法测算细胞凋亡率,用Western blotting法检测细胞增殖、凋亡相关蛋白与NF-κB信号通路相关蛋白[细胞周期蛋白D1(Cyclin D1)、半胱氨酸天冬氨酸蛋白水解酶3(Caspase-3)、NF-κB p65、磷酸化NF-κB p65(NF-κB p65)]表达。结果与对照组比较,5μmol/L安罗替尼组细胞活力差异无统计学意义(P>0.05),10、20μmol/L安罗替尼组、阳性药物组的细胞活力低(P均<0.05)。与对照组比较,各安罗替尼组、阳性药物组细胞增殖率、Cyclin D1、p-NF-κB p65蛋白表达低,而细胞凋亡率、Caspase-3蛋白表达高(P均<0.05);与阳性药物组比较,10μmol/L安罗替尼组细胞增殖率、Cyclin D1、p-NF-κB p65蛋白表达高(P均<0.05),而细胞凋亡率、Caspase-3蛋白表达低(P均<0.05),20μmol/L安罗替尼组与阳性药物组比较各指标差异无统计学意义(P均>0.05);与10μmol/L安罗替尼组比较,抑制剂组各指标变化更明显(P均<0.05)。结论安罗替尼可能通过抑制NF-κB通路信号抑制人脑胶质瘤细胞增殖,并促进其凋亡。Objective To investigate the effects of anlotinib on proliferation and apoptosis of human glioma cells(T98G cells)through nuclear transcription factor-κB(NF-κB)signaling pathway.Methods T98G cells were cultured in vitro and randomly divided into the control group,5μmol/L anlotinib group,10μmol/L anlotinib group,20μmol/L anlotinib group,positive drug group,and inhibitor group.Cells in the control group were not intervened,cells in the 5,10,and 20μmol/L anlotinib group were treated with 5,10 and 20μmol/L anlotinib,respectively,and cells in the positive drug group were treated with 50 mg/L 5-fluorouracil;in the inhibitor group,10μmol/L anlotinib+5μmol/L NF-κB pathway inhibitor BAY 11-7082 were added for intervention.Cell viability was measured by CCK-8,cell proliferation rate was measured by 5-acetylidene-2'deoxyuracil riboside method,and apoptosis rate was measured by Hoechst33258 staining.The expression levels of cell cycle proteins D1(CyclinD1),Caspase-3,NF-κB p65 and phosphorylated(p-)NF-κB p65 were detected by Western blotting.Results Compared with the control group,there was no significant difference in cell viability in the 5μmol/L anlotinib group(P>0.05),while the cell viability in the 10,20μmol/L anlotinib groups and positive drug group was lower(all P<0.05).Compared with the control group,the cell proliferation rates,and the expression levels of CyclinD1 and p-NF-κB p65 protein were lower,while the apoptosis rates and the expression levels of Caspase-3 protein were higher in each anlotinib group and positive drug group(all P<0.05).Compared with the positive drug group,the cell proliferation rate,the expression levels of CyclinD1 and P-NF-κB p65 protein in the 10μmol/L anrotinib group were higher(all P<0.05),while the apoptosis rate and the expression of Caspase-3 protein were lower(both P<0.05).There was no significant difference between 20μmol/L anlotinib group and positive drug group(P>0.05).Compared with the 10μmol/L anrotinib group,the changes of all indexes in the inhibitor group we

关 键 词：脑胶质瘤 安罗替尼 核因子ΚB信号通路 细胞增殖 细胞凋亡 

分 类 号：R739.41[医药卫生—肿瘤]