Hexafluoropropylene oxide trimer acid,a perfluorooctanoic acid alternative,induces cardiovascular toxicity in zebrafish embryos  

作　　者：Sujie Sun Li Zhang Xue Li Lu Zang Ling Huang Junquan Zeng Zigang Cao Xinjun Liao Zilin Zhong Huiqiang Lu Jianjun Chen 

机构地区：[1]Jiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases,Jiangxi Key Laboratory of Developmental Biology of Organs,Center for Clinical Research Center of the Affiliated Hospital of Jinggangshan University,Ji’an 343009,China [2]Translational Research Institute of Brain and Brain-like Intelligence,Shanghai Key Laboratory of Anesthesiology and Brain Functional Modulation,Department of Pediatrics,Shanghai Fourth People’s Hospital,School of Medicine,Tongji University,Shanghai 200434,China [3]State Environmental Protection Key Laboratory of Environmental Health Impact Assessment of Emerging Contaminants,School of Environmental Sciences and Engineering,Shanghai Jiao Tong University,Shanghai 200240,China [4]Department of Interventional and Vascular Surgery,Affiliated Hospital of Jinggangshan University,Ji’an 343009,China [5]Department of Internal Medicine and Hematology,Affiliated Hospital of Jinggangshan University,Ji’an 343009,China

出　　处：《Journal of Environmental Sciences》2024年第5期460-472,共13页环境科学学报（英文版）

基　　金：supported by the Ministry of Science and Technology of China(Nos.2021YFA1101300 and 2020YFA0112500);the National Key R&D Program of China(2018YFA0801000);the National Natural Science Foundation of China(No.32170853)。

摘　　要：As an increasingly used alternative to perfluorooctanoic acid(PFOA),hexafluoropropylene oxide trimer acid(HFPO-TA)has been widely detected in global water environments.However,little is known regarding its toxic effects on cardiovascular development.Here,zebrafish embryos were treated with egg water containing 0,60,120,or 240 mg/L HFPO-TA.Results showed that HFPO-TA treatment led to a significant reduction in both larval survival percentage and heart rate.Furthermore,HFPO-TA exposure caused severe pericardial edema and elongation of the sinus venous to bulbus arteriosus distance(SV-BA)in Tg(myl7:GFP)transgenic larvae,disrupting the expression of genes involved in heart development and thus causing abnormal heart looping.Obvious sprouting angiogenesis was observed in the 120 and 240 mg/L exposed Tg(fli:GFP)transgenic larvae.HFPO-TA treatment also impacted the mRNA levels of genes involved in the vascular endothelial growth factor(VEGF)pathway and embryonic vascular development.HFPO-TA exposure significantly decreased erythrocyte number in Tg(gata1:DsRed)transgenic embryos and influenced gene expression associated with the heme metabolism pathway.HFPO-TA also induced oxidative stress and altered the transcriptional levels of genes related to cell cycle and apoptosis,inhibiting cell proliferation while promoting apoptosis.Therefore,HFPO-TA exposure may induce abnormal development of the cardiovascular and hematopoietic systems in zebrafish embryos,suggesting it may not be a suitable or safe alternative for PFOA.

关 键 词：Hexafluoropropylene oxide trimer ACID Heart development Vascular development HEMOPOIESIS Zebrafish embryos 

分 类 号：X171.5[环境科学与工程—环境科学]