茶树炭疽菌N425全基因组测序与基因功能注释  

Sequence and Annotation of Colletotrichum fructicola N425 Genome on Tea Plant

作　　者：张承康 周子文 郭田龙 彭成彬 陈美霞 刘伟 ZHANG Chengkang;ZHOU Ziwen;GUO Tianlong;PENG Chengbin;CHEN Meixia;LIU Wei(Provincial demonstration base for industry university research cooperation,College of Life Science,Ningde Normal University,Ningde,Fujian 352100,China;Key Laboratory of Biopesticide and Chemical Biology,Ministry of Education,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]宁德师范学院生命科学学院省级产学研合作示范基地,福建宁德352100 [2]福建农林大学生物农药与化学生物学教育部重点实验室,福建福州350002

出　　处：《福建农业学报》2023年第12期1437-1444,共8页Fujian Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(32001853);生物农药与化学生物学教育部重点实验室开放课题(Keylab2020-05);宁德师范学院科研项目(2020Y02、2022T01)。

摘　　要：【目的】对茶树炭疽菌N425进行全基因组测序和基因功能注释并从中初筛出致病相关基因。【方法】通过Illumina HiSeq2500 PE150平台对N425菌株进行全基因组测序和组装,并利用NR、KEGG、KOG等功能基因数据库进行基因预测和功能注释。【结果】茶树炭疽菌N425基因组全长约56.3 Mbp,G+C含量为53.2%,共预测出10 157个蛋白编码基因和若干各类非蛋白编码序列。其中,1 356个基因在病原与宿主互作数据库(PHI)中得到注释,包括140个注释为致病力丧失的基因;720个基因在碳水化合物酶数据库(CAZy)中得到注释;302个基因被预测为次生代谢物相关基因。这些基因中有涉及cAMP-PKA、MAPK等信号通路以及降解宿主细胞壁等致病相关过程,是潜在的致病相关基因。【结论】本研究成功获得茶树炭疽菌N425全基因组序列和基因功能注释,并从中挖掘出潜在致病相关基因,为后续展开对茶树炭疽菌侵染茶树的致病分子机制研究奠定基础。【Objective】Sequence and annotation of Colletotrichum fructicola N425 genome from a diseased tea plant were determined,and primary virulence-related genes identified.【Method】Whole genome of N425 was sequenced and assembled using the Illumina HiSeq2500 PE150 platform.Predicted protein structure and functional annotation of the genes were obtained using the NR,KEGG,and KOG databases.【Results】The genome was approximately 56.3 Mbp in length with 53.2%of G+C,10157 protein-coding genes,and several types of non-protein coding sequences.Of the genes,1356 were annotated in the PHI database that included 140 as the loss of pathogenicity,720 in the CAZy database,and 302 related to the secondary metabolites.Some of these genes were involved in the cAMP-PKA and MAPK cascade signaling pathways as well as other pathogenic processes,such as host cell wall degradation,which could be responsible for the virulence on tea plants.【Conclusion】The sequence and annotation of whole C.fructicola N425 genome were successfully obtained,and probable virulence-related genes identified.

关 键 词：茶树炭疽病 基因组测序 基因注释 致病相关基因 

分 类 号：S436[农业科学—农业昆虫与害虫防治]