漆酶-原儿茶醛交联鱼皮胶原蛋白水凝胶的研究  

Study on the Collagen Hydrogel of Fish Skin via Laccase-Protocatechuic Aldehyde Cross-linking

作　　者：杨长凯 李国英[1,2] YANG Changkai;LI Guoying(Key Laboratory of Leather Chemistry and Engineering of Ministry of Education,Sichuan University,Chengdu 610065,China;National Engineering Laboratory for Clean Technology of Leather Manufacture,Sichuan University,Chengdu 610065,China)

机构地区：[1]四川大学皮革化学与工程教育部重点实验室,四川成都610065 [2]四川大学制革清洁技术国家工程实验室,四川成都610065

出　　处：《皮革科学与工程》2024年第2期1-6,46,共7页Leather Science and Engineering

基　　金：国家自然科学基金资助项目(22078206)。

摘　　要：与哺乳动物蛋白相比,鱼皮胶原蛋白未受到克雅氏病和牛海绵状脑病流行的限制,具有较大的应用潜力,然而较差的力学性能和耐降解性质限制了鱼皮胶原的应用。论文探究了漆酶(LAC)和原儿茶醛(PAL)交联制备胶原蛋白水凝胶的交联机理,凝胶微观结构,力学性能和耐降解性质。结果表明,漆酶能够交联胶原成胶。同时,原儿茶醛的引入增强了漆酶交联胶原的作用,提升了胶原蛋白水凝胶的结构稳定性和耐降解性质。LAC-PAL交联胶原是一种构建胶原蛋白水凝胶的有效策略。Fish skin collagen has greater application potential than mammalian proteins because it is not limited by the prevalence of Creutzfeldt-Jakob disease and bovine spongiform encephalopathy.However,it is restricted by its poor mechanical properties and poor resistance to degradation.This work investigated the cross-linking mechanism,gel microstructure,mechanical properties and degradation resistance of collagen hydrogels prepared by the cross-linking of laccase(LAC)with protocatechuic aldehyde(PAL).Results showed that LAC could cross-link collagen to form a hydrogel.Meanwhile,the introduction of PAL enhanced the role of LAC in cross-linking collagen and improved the structural stability and degradation resistance of the resulting collagen hydrogels.The collagen cross-linked via LAC-PAL is an effective strategy for constructing collagen hydrogels.

关 键 词：水凝胶 鱼皮 胶原 漆酶 原儿茶醛 

分 类 号：TS512[轻工技术与工程—皮革化学与工程]