子宫肌瘤患者血清lncRNA MIAT和lncRNA XIST的表达及临床意义  

Expression and clinical significance of serum lncRNA MIAT and lncRNA XIST in patients with uterine myoma

作　　者：李玉萍 马会[2] 禹航 LI Yuping;MA Hui;YU Hang(Department of Gynecology,Zhangjiakou Maternal and Child Health Hospital,Zhangjiakou 075000,China;Department of Gynecology,The First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,China)

机构地区：[1]张家口市妇幼保健院妇科,河北张家口075000 [2]河北北方学院附属第一医院妇科,河北张家口075000

出　　处：《安徽医学》2024年第2期174-179,共6页Anhui Medical Journal

摘　　要：目的 检测子宫肌瘤患者血清长链非编码RNA(lncRNA)心肌梗死相关转录本(MIAT)、lncRNA X染色体失活特异转录本(XIST)的水平,并探究二者与子宫肌瘤的关系以及对子宫肌瘤的诊断价值。方法 选取2018年5月至2022年5月在张家口市妇幼保健院就诊的120例子宫肌瘤患者作为子宫肌瘤组,选择同时期在本院进行体检的120例健康体检者作为对照组。比较两组研究对象的基线资料;实时荧光定量聚合酶链式反应(qRT-PCR)检测两组研究对象血清中lncRNA MIAT、lncRNA XIST表达水平;Pear?son相关性分析子宫肌瘤患者血清中lncRNA MIAT与lncRNA XIST表达水平的相关性;多因素logistic回归分析子宫肌瘤患病的影响因素;采用受试者工作特征(ROC)曲线评价血清lncRNA MIAT、lncRNA XIST对子宫肌瘤的诊断价值。结果 两组研究对象肌瘤最大径、肌瘤数目、有无乳腺增生、流产次数、有无妇科疾病比较,差异有统计学意义(P<0.05);与对照组比较,子宫肌瘤组血清中ln?cRNA MIAT、lncRNA XIST水平升高,差异有统计学意义(P<0.05);肌瘤最大径≥5 cm、肌瘤数目≥2个、有乳腺增生、流产次数≥2次、有妇科疾病的子宫肌瘤患者血清中lncRNA MIAT、lncRNA XIST表达水平明显高于肌瘤最大径<5 cm、肌瘤数目<2个、无乳腺增生、流产次数<2次、无妇科疾病的子宫肌瘤患者,差异有统计学意义(P<0.05);Pearson相关性分析显示,子宫肌瘤患者血清中lncRNA MIAT、lncRNA XIST表达水平呈正相关(r=0.468,P<0.001);多因素logistic回归分析结果显示,lncRNA MIAT≥1.45(OR=1.678,95%CI:1.342~2.098)、lncRNA XIST≥1.35(OR=1.315,95%CI:1.033~1.673)是子宫肌瘤发生的危险因素(P<0.05);血清lncRNA MIAT、lncRNA XIST诊断子宫肌瘤的曲线下面积(AUC)分别为0.817、0.776,特异度分别为91.7%、86.7%,灵敏度分别为62.5%、71.7%,截断值分别为1.45、1.35,两者联合诊断子宫肌瘤的AUC为0.846,特异度为97.5%,灵敏度为61.2%。结论 子Objective To detect the levels of serum long non-coding RNA(lncRNA) myocardial infarction associated transcript(MIAT) and lncRNA X chromosome inactivation specific transcript(XIST) in patients with uterine myoma,and to explore the relationship be?tween them and uterine myoma and their diagnostic value for uterine myoma.Methods A total of 120 patients with uterine myoma who were treated in Zhangjiakou Maternal and Child Health Hospital from May 2018 to May 2022 were selected as the uterine myoma group;meanwhile,120 healthy people who had physical examination in our hospital were regarded as the control group.Baseline data were compared between the two groups of subjects.Real-time fluorescence quantitative polymerase chain reaction(qRT-PCR) was applied to detect the expression levels of lncRNA MIAT and lncRNA XIST in serum from both groups.Pearson correlation was applied to analyze the correlation between the expres?sion levels of lncRNA MIAT and lncRNA XIST in serum of patients with uterine myoma.Multivariate Logistic regression analysis was applied to analyze the influencing factors of uterine myoma.The subject working characteristic(ROC) curve was plotted to evaluate the diagnostic value of serum lncRNA MIAT and lncRNA XIST in uterine myoma.Results There were statistically significant differences in the largest diameter of fibroids,the number of fibroids,the presence of breast hyperplasia,the number of abortion and the presence of gynecological diseases between the two groups(P<0.05).Compared with the control group,the serum levels of lncRNA MIAT and lncRNA XIST in the uterine myoma group were higher(P<0.05);the expression levels of lncRNA MIAT and lncRNA XIST in serum of patients with myoma with maximum diameter of myoma ≥5,number of myoma ≥2,hyperplasia of mammary gland,number of abortions ≥2,and gynecological diseases were obviously higher than those of patients with myoma with maximum diameter of myoma <5,number of myoma <2,no hyperplasia of mammary gland,number of abortions <2,and no gynecological dis

关 键 词：子宫肌瘤 长链非编码RNA 心肌梗死相关转录本 X染色体失活特异转录本 

分 类 号：R737.33[医药卫生—肿瘤]