慢性牙周炎患者血清TFF3,FKN水平与牙周病变程度及牙周临床指标的相关性分析  

Correlation analysis of serum TFF3 and FKN levels with the degree of periodontal disease and clinical periodontal indicators in patients with chronic periodontitis

作　　者：刘喜玲 雷祎 严晖 LIU Xi-ling;LEI Yi;YAN Hui(Department of Stomatology,The Ninth Hospital of Wuhan,Hubei Wuhan 430080,China;Department of Stomatology,Xiling Hospital,Yichang Central People's Hospital,Hubei Yichang 443000,China)

机构地区：[1]武汉市第九医院口腔科,湖北武汉430080 [2]宜昌市中心人民医院西陵院区口腔科,湖北宜昌443000

出　　处：《临床口腔医学杂志》2024年第1期30-34,共5页Journal of Clinical Stomatology

基　　金：湖北省卫生健康委科研项目(WJ2017MJ87)。

摘　　要：目的:探究慢性牙周炎(chronic periodontitis,CP)患者血清中三叶因子3(trefoil factor 3,TFF3)、不规则趋化因子(fractalkine,FKN)表达水平与牙周病变程度及牙周临床指标的相关性分析。方法:前瞻性选取2023年1月~6月期间在本院口腔科就诊的120例CP患者,男56例,女64例,根据牙周病变程度分为轻度组(35例)、中度组(48例)、重度组(37例)。同时随机选取120例就诊的牙周组织健康者记为对照组,男67例,女53例。酶联免疫吸附实验(ELISA)检测TFF3、FKN表达水平;Pearson相关性分析法分析TFF3、FKN表达水平与牙周临床指标的相关性;多因素Logistic回归分析重度CP发生的影响因素;受试者工作特征(receiver operating characteristic,ROC)曲线评估TFF3、FKN对CP严重程度的诊断价值。结果:CP患者血清TFF3、FKN表达水平显著高于对照组(P<0.05),且随着病情严重程度增加,二者表达水平逐渐升高(P<0.05);CP患者血清TFF3、FKN表达水平与探诊深度(PD)、附着丧失(AL)、菌斑指数(PLI)和出血指数(BI)等牙周临床指标呈正相关(P<0.05)。TFF3、FKN诊断中度CP的曲线下面积(the area under the ROC curve,AUC)为0.807(95%CI 0.715~0.900)、0.824(95%CI 0.737~0.911),两者联合诊断的AUC为0.913(95%CI 0.852~0.973),二者联合诊断中度CP优于单独诊断(P=0.009,P=0.017)。TFF3、FKN诊断重度CP的AUC为0.798(95%CI 0.702~0.893)、0.793(95%CI 0.691~0.895),两者联合诊断的AUC为0.900(95%CI 0.826~0.975),二者联合诊断重度CP优于单独诊断(P=0.006,P=0.009)。结论:随着CP患者病情严重程度的增加,血清中TFF3、FKN表达水平逐渐升高,且与牙周临床指标呈正相关,TFF3、FKN联合诊断CP患者病情严重程度具有较高价值,可作为早期诊断指标。Objective:To investigate the correlation between the expression levels of trefoil factor 3(TFF3)and fractalkine(FKN)in the serum of patients with chronic periodontitis(CP)and the degree of periodontal disease and clinical periodontal indicators.Methods:This study prospectively selected 120 CP patients who visited our dental department from January to June 2023 as the CP group,including 56 males and 64 females.They were grouped into mild group(35 cases),moderate group(48 cases),and severe group(37 cases)based on the degree of periodontal disease.Meantime,120 healthy individuals with periodontal tissue who visited the dental department of our hospital were randomly selected as the control group,including 67 males and 53 females.Enzyme linked immunosorbent assay(ELISA)was applied to detect the expression levels of TFF3 and FKN.Pearson correlation analysis method was applied to analyze the correlation between TFF3,FKN expression levels and periodontal clinical indicators.Multivariate Logistic regression was applied to analyze the influencing factors of severe CP occurrence,receiver operating characteristic(ROC)curve was applied to evaluate the diagnostic value of TFF3 and FKN for CP severity.Results:The expression levels of TFF3 and FKN in the serum of CP patients were obviously higher than those in the control group(P<0.05),and their expression levels gradually increased with the severity of the disease(P<0.05).The expression levels of TFF3 and FKN in the serum of CP patients were positively correlated with periodontal clinical indicators such as probing depth(PD),attachment loss(AL),plaque index(PLI),and bleeding index(BI)(P<0.05).The area under the curve(AUC)for the diagnosis of moderate CP by TFF3 and FKN was 0.807(95%CI 0.715~0.900)and 0.824(95%CI 0.737~0.911),respectively,the AUC for the combined diagnosis of the two was 0.913(95%CI 0.852~0.973),and the combined diagnosis of the two was better than that of serum TFF3 and FKN alone(P=0.009,P=0.017).The diagnosis of severe CP with TFF3 and FKN was 0.798(95%CI 0.7

关 键 词：慢性牙周炎 三叶因子3 不规则趋化因子 牙周临床指标 

分 类 号：R781.6[医药卫生—口腔医学] R782.1[医药卫生—临床医学]