辣木叶标准汤剂的煎煮工艺优化及质量标准建立  

Optimization of Decoction Technology and Establishment of Quality Standard for Moringa oleifera Leaf Standard Decoction

作　　者：宋亚南 王云[1] 杨洪军[1,2] 贾哲 张村[1] SONG Yanan;WANG Yun;YANG Hongjun;JIA Zhe;ZHANG Cun(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]中国中医科学院,北京100700

出　　处：《中国实验方剂学杂志》2024年第6期151-159,共9页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：云南省重大科技专项计划项目(2017ZF004)。

摘　　要：目的:基于响应面法结合主成分分析,优选辣木叶标准汤剂最佳煎煮工艺,建立其多指标质量评价体系,为辣木叶标准汤剂的质量控制提供科学依据。方法:采用响应面法结合主成分分析法,以8个特征峰的相对峰面积及出膏率作为评价指标,优选出最佳煎煮工艺。在此基础上,以15批标准汤剂为研究对象,以高效液相色谱法(HPLC)特征图谱、多指标成分(新绿原酸、L-色氨酸、隐绿原酸、维采宁-2、异槲皮素、紫云英苷)含量测定、大类成分(总黄酮、总有机酸、总多糖、总α-氨基酸、总芥子碱)含量测定、出膏率、比重、pH等多指标开展其质量标准研究。结果:优化得到的最佳煎煮工艺是取辣木叶100.00 g,浸泡30 min,煎煮2次,头煎12倍量水,煎煮30 min;二煎10倍量水,煎煮20 min。以x±30%限定各数值范围,含生药量0.2g·mL^(-1)的辣木叶标准汤剂,其比重为0.722~1.340、pH为3.86~7.16、出膏率为23.1%~42.9%、醇溶性浸出物为8.26%~15.34%;按标准汤剂干燥品计,新绿原酸、L-色氨酸、隐绿原酸、维采宁-2、异槲皮素、紫云英苷的质量分数分别为1.99~3.69、1.20~2.22、1.44~2.67、0.53~0.99、2.45~4.55、1.22~2.26 mg·g^(-1),相对于药材的转移率分别为34.37%~63.83%、62.43%~115.94%、64.65%~120.06%、56.98%~105.82%、37.46%~69.57%、41.81%~77.64%;总黄酮、总有机酸、总多糖、总α-氨基酸、总芥子碱的质量分数分别为10.19~18.92、11.82~21.96、94.07~174.71、42.69~79.27、9.55~17.73mg·g^(-1),相对于药材的转移率为25.72%~47.77%、41.78%~77.59%、64.90%~120.54%、42.30%~78.57%、34.99%~64.99%。结论:优选的辣木叶标准汤剂煎煮工艺稳定可行,建立的多指标质量评价体系可为该标准汤剂的质量控制奠定基础。Objective:Based on response surface methodology combined with principal component analysis(PCA),the optimal decocting process of Moringa oleifera leaf standard decoction was optimized,and its multi-index quality evaluation system was established,in order to provide scientific basis for the quality control of this standard decoction.Method:Response surface methodology and PCA were used to optimize the decoction process by taking the relative peak areas of 8 characteristic peaks and dry extract yield as indexes.Based on this,the quality of 15 batches of the standard decoction was evaluated by high performance liquid chromatography(HPLC)characteristic chromatogram,determination of major components(neochlorogenic acid,L-tryptophan,cryptochlorogenic acid,vicenin-2,isoquercetin,astragalin),determination of active parts(total flavonoids,total organic acids,total polysaccharides,totalα-amino acids,total sinapine),dry extract yield,specific gravity and pH.Result:The optimal decocting process was to soak M.oleifera leaves(100.00 g)for 30 min and decoct twice with the first decoction of 12 times the amount of water for 30 min and the second decoction of 10 times the amount of water for 20 min.Standard decoction containing 0.2 g·mL^(-1) of crude drug was defined by x±30%,the specific gravity was 0.722-1.340,pH was 3.86-7.16,dry extract yield was 23.1%-42.9%,and the alcohol-soluble extract content was 8.26%-15.34%.Calculated according to the dried products of the standard decoction,the contents of neochlorogenic acid,L-tryptophan,cryptochlorogenic acid,vicenin-2,isoquercetin and astragalin were 1.99-3.69,1.20-2.22,1.44-2.67,0.53-0.99,2.45-4.55,1.22-2.26 mg·g^(-1),the relative transfer rates relative to the herbs were 34.37%-63.83%,62.43%-115.94%,64.65%-120.06%,56.98%-105.82%,37.46%-69.57%,41.81%-77.64%,respectively.The contents of total flavonoids,total organic acids,total polysaccharides,totalα-amino acids,total sinapine were 10.19-18.92,11.82-21.96,94.07-174.71,42.69-79.27,9.55-17.73 mg·g^(-1),the relative transfer ra

关 键 词：辣木叶 标准汤剂 高效液相色谱法(HPLC) 质量标准 响应面法 主成分分析(PCA) 煎煮工艺 

分 类 号：R22[医药卫生—中医基础理论] C37[医药卫生—中医学] R28[社会学] O657[理学—分析化学]