水稻茎秆细胞壁相关组分含量QTL定位及候选基因分析  

Mapping of QTL for Cell Wall Related Components in Rice Stem and Analysis of Candidate Genes

作　　者：贾绮玮 钟芊芊 顾育嘉 陆天麒 李玮 杨帅[1] 朱超宇 胡程翔 李三峰[2] 王跃星[2] 饶玉春[1] Qiwei Jia;Qianqian Zhong;Yujia Gu;Tianqi Lu;Wei Li;Shuai Yang;Chaoyu Zhu;Chengxiang Hu;Sanfeng Li;Yuexing Wang;Yuchun Rao(College of Life Sciences,Zhejiang Normal University,Jinhua 321004,China;National Key Laboratory of Rice Biological Breeding,China National Rice Research Institute,Hangzhou 310006,China)

机构地区：[1]浙江师范大学生命科学学院,金华321004 [2]中国水稻研究所,水稻生物育种全国重点实验室,杭州310006

出　　处：《植物学报》2023年第6期882-892,共11页Chinese Bulletin of Botany

基　　金：浙江省自然科学基金重点项目(No.LZ23C130003);浙江省大学生科技创新活动计划暨新苗人才计划(No.2023R404040,No.2023R404041)。

摘　　要：水稻(Oryza sativa)倒伏是制约其生产的主要因素之一,而茎秆的机械强度影响水稻抗倒伏能力,且与茎秆细胞壁相关组分含量密切相关。通过调控水稻茎秆细胞壁相关组分含量提高水稻抗倒伏能力,是提高水稻产量与品质的有效途径。该研究用籼稻品种华占(O.sativa subsp.indica cv.‘HZ’)和粳稻品种热研2号(O.sativa subsp.japonica cv.‘Nekken2’)杂交获得F1代,经连续多代自交得到120个重组自交系(RILs)群体,并以此构建遗传连锁图谱。基于构建的高密度遗传图谱,对水稻茎秆细胞壁中纤维素、半纤维素和木质素含量相关QTLs进行定位,结果共检测到4个与纤维素含量相关的QTLs、12个与半纤维素含量相关的QTLs和8个与木质素含量相关的QTLs。对检测到的QTLs区间进行候选基因分析,共筛选到13个候选基因。利用q RT-PCR检测候选基因的表达水平,结果表明除LOC_Os02g58590和LOC_Os12g41720外,其余候选基因的表达量在双亲间均存在显著差异。研究结果为挖掘调控水稻茎秆机械强度的基因,进而筛选和培育抗倒伏能力强的水稻品种奠定了重要基础。Lodging of rice is one of the main factors reducing rice yield.The mechanical strength of stem affects lodging resistance of rice and is closely related to the content of stem cell wall related components.Improving lodging resistance of rice by regulating the related components in the cell wall of stem is an effective way to improve the yield and quality of rice.In this study,indica rice variety Huazhan(Oryza sativa subsp.indica cv.‘HZ’)and japonica rice variety Nekken2(O.sativa subsp.japonica cv.‘Nekken2’)were crossed to obtain F1 generation.A total of 120 RILs(recombinant inbred lines)population were obtained by successive multigeneration self-crossing and the genetic linkage map was constructed.Based on the constructed high-density genetic map,the QTLs related to the content of cellulose,hemicellulose,and lignin in the cell wall of rice stem were located and analyzed.The results showed that 4 QTLs related to cellulose,12 QTLs related to hemicellulose,and 8 QTLs related to lignin.At the same time,candidate genes analyses were conducted on the detected QTLs’intervals,and a total of 13 candidate genes were screened.The expression levels of candidate genes were detected by qRT-PCR.Except for LOC_Os02g58590 and LOC_Os12g41720,the other candidate genes showed significant differences between parents.The results laid an important foundation for exploring genes that regulate the mechanical strength of rice stems,further screening and breeding rice varieties with strong lodging resistance.

关 键 词：水稻 茎秆机械强度 QTL定位 候选基因 抗倒伏 

分 类 号：S511[农业科学—作物学]