3种桑螵蛸炮制前后抗氧化活性及免疫学研究  

Antioxidative activity and immunological study of three kinds of mantidis ootheca before and after processing

作　　者：刘雨冬 冀国鑫 王仁广 胡德 闫茂颖 王淑敏[1] LIU Yu-dong;JI Guo-xin;WANG Ren-guang;HU De;YAN Mao-ying;WANG Shu-min(Changchun University of Chinese Medicine,Changchun 130117,China)

机构地区：[1]长春中医药大学,长春130117

出　　处：《中国新药杂志》2024年第2期168-176,共9页Chinese Journal of New Drugs

基　　金：吉林省中医药科技项目(JLPZGF-2020-085)。

摘　　要：目的:比较3种桑螵蛸生品、盐制品对小鼠免疫功能和体外抗氧化能力的影响.方法:实验采用1,1-二苯基-2-三硝基苯肼(DPPH)和2,2-联氮-(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)2种方法,考察3种桑螵蛸炮制前后体外抗氧化活性的变化规律.通过腹腔注射环磷酰胺建立小鼠免疫力低下模型,测定小鼠体重变化率、免疫器官脏器指数和肾脏指数.通过酶联免疫法检测血清中白细胞介素2(IL-2)、肿瘤坏死因子α(TNF-α)、干扰素γ(IFN-γ)、免疫球蛋白M(lgM)的水平.采用苏木素-伊红(HE)染色法观察各组小鼠胸腺、脾脏、肾脏病理改变情况.用Western blot法检测小鼠脾组织中核转录因子-κB(nuclear factor-κB,NF-κB)(P65),NF-κB(PP65),核因子κB抑制蛋白(IKB-α)的相对表达量.结果:体外抗氧化结果表明,3种桑螵蛸及盐制品均具有一定的自由基清除效果.盐制团螵蛸体外抗氧化活性最强,其清除DPPH,ABTS自由基的半数抑制浓度(IC50)分别为0.507,0.705 mg·mL-1.免疫活性结果表明,3种桑螵蛸及盐制品对于免疫力低下模型小鼠体重变化率、胸腺指数、脾脏指数均有不同程度的提高,以盐制团螵蛸效果最佳,与模型组体重变化率、脾脏指数相比差异极显著(P<0.05,P<0.001,P<0.01).模型组细胞因子IL-2,TNF-α,IFN-γ及免疫球蛋白IgM含量与对照组比均下降(P<0.001).与模型组比较,团螵蛸生品及盐制品IL-2的含量回调,具有显著性差异(P<0.05),3种桑螵蛸生品及盐制品TNF-α的含量均升高,具有显著性差异(P<0.05,P<0.01,P<0.001),盐制团螵蛸、盐制长螵蛸及团螵蛸的IFN-γ的含量升高,具有显著性差异(P<0.05,P<0.01),团螵蛸与盐制团螵蛸IgM的含量升高,具有显著性差异(P<0.01).盐制团螵蛸可以改善胸腺、脾脏、肾脏的损伤,在3种桑螵蛸及炮制品中效果最优.桑螵蛸可使NF-κB(P65),NF-κB(PP65),IKB-α的相对表达回调到正常状态(P<0.001,P<0.0001).结论:3种桑螵蛸生品、�Objective:To compare the effects of the raw and salt-processed products of three kinds of mantidis ootheca on immune function of mice and in vitro antioxidant activity.Methods:1,1-diphenyl-2-picrylhydrazyl and 2,2'-azinobis-3-ethylbenzthiazoline-6-sul phonate methods were used to investigate the changes of antioxidant activity in vitro before and after salt-processing of three kinds of mantidis ootheca.The immunocompromised mouse model was established by intraperitoneal injection of cyclophosphamide,and the weight change rate,immune organ index and kidney index were determined.Serum levels of interleukin-2(IL-2),tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ),and immunoglobulin M(lgM)were detected by enzyme-linked immunosorbent assay.Hematoxylin-eosin(HE)staining was used to observe the pathological changes of the thymus,spleen and kidney of the mice in each group.The relative expressions of nuclear transcription factor-κB p65[nuclear factor kappa-B p65,NF-κB(P65)],NF-κB(PP65),and IKB-αin mouse spleen tissues were detected by Western blot.Results:The in vitro antioxidant assay showed that the three kinds of mantidis ootheca and their salt-processed products had certain free radical scavenging effects.The anti-oxidative activity of salt-processed Tenodera sinensis Saussure was the strongest in vitro,and its IC50 values for scavenging DPPH and ABTS free radicals were 0.507 and 0.705 mg·mL-1,respectively.The results of immune activity study showed that the three kinds of mantidis ootheca and salt-processed products could improve the body weight change rate,thymus index and spleen index of the immunocompromised model mice to varying degrees.Salt-processed Tenodera sinensis Saussure had the best effect.The body weight change rate and spleen index were significantly different(P<0.05,P<0.001,P<0.01).The contents of cytokines IL-2,TNF-α,IFN-γand immunoglobulin IgM in the model group were decreased to different degrees(P<0.001).Compared with the model group,the contents of IL-2 in the raw and salt-processed

关 键 词：桑螵蛸 环磷酰胺 免疫调节 抗氧化活性 

分 类 号：R965.1[医药卫生—药理学] R975.5[医药卫生—药学]