超小粒径的纳米Ag协同Cu基铁氧体复合材料的制备及其对金黄色葡萄球菌抑菌机制研究  

Preparation of ultrasmall-sized Ag in synergy with Cu-based ferrite composite and its antibacterial activity against Staphylococcus aureus

作　　者：郭少波 吴迎花 陈惠惠 徐海涛 史娟[1,2] 刘智峰 季晓晖[1,2] 张天雷 GUO ShaoBo;WU YingHua;CHEN HuiHui;XU HaiTao;SHI Juan;LIU ZhiFeng;JI XiaoHui;ZHANG TianLei(School of Chemistry and Environmental Science,Shaanxi University of Technology,Hanzhong 723000,China;Shaanxi Province Key Laboratory of Catalytic Foundation and Application,Hanzhong 723000,China)

机构地区：[1]陕西理工大学化学与环境科学学院,汉中723000 [2]陕西省催化基础与应用重点实验室,汉中723000

出　　处：《中国科学：生命科学》2024年第2期338-348,共11页Scientia Sinica(Vitae)

基　　金：秦巴生物资源与生态环境国家重点实验室科研基金(批准号:SXS-2105);陕西省教育厅项目(批准号:22JK-0317);陕西省自然科学基金(批准号:2023-JC-QN-0162,2023-YBSF-334);陕西理工大学基础研究基金(批准号:SLGKYXM2208)资助。

摘　　要：超小粒径的纳米银(Ag)可通过细菌细胞壁,进入细菌内环境后对细菌造成不可逆损伤,因而具备较强的抑菌活性.但是超小粒径的纳米Ag易团聚,且单独使用时毒性较强,这些缺点限制了其应用.本研究利用生物相容性较高的Fe和Cu化合物制备了Fe_(3)O_(4)/Cu/CuO载体(F),将介孔(mesoporous, m)ZrO_(2)包覆在F上合成纳米Fe_(3)O_(4)/Cu/Cu O@mZrO2(FZ),然后将~3 nm的Ag负载在其表面,制备出核壳型纳米Fe_(3)O_(4)/Cu/CuO@mZrO_(2)@Ag(FZA)复合材料. FZA不仅可解决纳米Ag团聚的问题,而且通过Fenton反应缓释的ROS(·OH)和Cu^(2+)可协同纳米Ag具备较强的抑菌性能.研究结果表明, FZA在40 min,150μg/mL时对金黄色葡萄球菌(S. aureus)和耐甲氧西林金黄色葡萄球菌(T-S. aureus)的抑菌率为99.99%,可有效破坏细菌细胞壁,导致核酸和细胞质泄露而诱导细菌凋亡.另外, FZA对体细胞的毒性相比单质Ag降低了1/2,因此可被应用于敷料研究.Nano-silver (Ag) with ultra-small particle sizes have strong antibacterial activity because it can pass through the bacterial cell walland enter the internal environment, causing irreversible damages to bacteria, but the disadvantages such as easy agglomeration andhigh toxicity limit its application. In this study, Fe_(3)O_(4)/Cu/CuO carriers (F) were prepared using Fe and Cu compounds with highbiocompatibility, and mesoporous (m) ZrO_(2) was coated on the surface of the carriers to synthesize Fe_(3)O_(4)/Cu/CuO@mZrO_(2) nanocomposites (FZ). Then ~3 nm Ag was loaded on the surface of FZ to prepare core-shell Fe_(3)O_(4)/Cu/CuO@mZrO_(2)@Ag (FZA)composite materials, which not only solved the problem of Ag agglomeration, but produced strong antibacterial properties with ROS(-OH) and Cu^(2+) released by Fenton reaction. The results showed that 150 μg/mL FZA inhibited 99.99% of Staphylococcus aureus (S.aureus) and Methicillin-resistant Staphylococcus aureus (T-S. aureus) after 40 min treatment. It could effectively disrupt the bacterialcell wall and lead to nucleic acid and cytoplasmic leakage, which consequently induced bacterial apoptosis. In the meantime, thetoxicity of FZA to somatic cells was reduced by 1/2 when compared with that of single Ag. Therefore, FZA could be used in dressingstudies on account of its strong antibacterial properties.

关 键 词：抑菌 AG 复合材料 抑菌机制 金黄色葡萄球菌 

分 类 号：R318.08[医药卫生—生物医学工程] TB33[医药卫生—基础医学]