MED8表达与肝癌临床病理特征及其预后的相关性分析  

Correlation Analysis of MED8 Expression and Clinicopathological Features and Prognosis in Hepatocellular Carcinoma

作　　者：吴爽 张涛元 李俏 曹三成[1] 王增国[1] 祝撷英[1] WU Shuang;ZHANG Taoyuan;LI Qiao(Xi'an Children's Hospital,Xi'an,710003)

机构地区：[1]陕西省西安市儿童医院,710003 [2]西京医院,710003

出　　处：《实用癌症杂志》2024年第3期411-416,共6页The Practical Journal of Cancer

摘　　要：目的检测中介体复合物亚基8(mediator complex subunit 8,MED8)在肝细胞癌(hepatocellular carcinoma,HCC)中的表达情况,探讨MED8对HCC的诊断、预后价值及其调控HCC的可能机制。方法从TCGA数据库下载HCC RNAseq数据以及临床数据,比较HCC癌旁组织和癌组织中MED8的表达差异;Western blot检测肝癌细胞HepG2及正常肝细胞QSG-7701中MED8的表达情况;通过DESeq2包和clusterProfiler包对含有MED8高、低表达组的数据进行表达差异分析及gene ontology(GO)富集分析;免疫组化检测补体经典途径相关蛋白C1qc的表达情况及其与MED8的关系。生存曲线、ROC曲线分析MED8表达与肝癌临床病理特征的相关性与预后价值。结果MED8在HCC癌组织及肝癌细胞中呈高表达。富集分析显示与MED8相互作用的蛋白主要参与补体的激活和离子反应等生物过程;而补体经典途径相关蛋白C1qc在肝癌组织中上调且与MED8表达呈正相关。临床相关性分析显示:MED8高表达与HCCT分期、病理分期、组织学分级密切相关;生存曲线结果显示:HCC高表达MED8患者总生存率及疾病相关存活率低;ROC曲线结果标明MED8的曲线下面积为0.817,提示MED8可能作为HCC潜在的诊断分子标志物。结论MED8与肝癌患者的临床分期及预后密切相关,其可能通过补体激活途径影响患者预后。Objective To investigate the expression,the diagnostic and the prognostic value of mediator complex subunit 8(MED8)in hepatocellular carcinoma(HCC)as well as its possible mechanism in regulating HCC.Methods HCC RNASeq data and clinical information were downloaded from the TCGA database.The expression of MED8 in HCC cancer tissue and the paired paracancerous samples were analyzed.Western blot was used to detect the expression of MED8 in HepG2 cells and QSG-7701 normal liver cells.DESeq2 package and clusterProfiler package were used to analyze the expression difference and gene ontology(GO)enrichment analysis of the data in MED8 high and low expression groups.Immunohistochemistry was used to detect the expression of the classical complement pathway-related protein C1qc and its relationship with MED8.The correlation and prognostic value of MED8 level with clinicopathological characteristics were analyzed by survival curve and ROC curve.Results MED8 was highly expressed in HCC cancer tissues and liver cancer cells.Enrichment analysis showed that proteins interacting with MED8 were mainly involved in biological processes such as complement activation and ion response;while the classical complement pathway-related protein C1qc was up-regulated in liver cancer samples and positively correlated with MED8 expression.High expression of MED8 was closely related to T stage,pathological stage and histological grade of HCC.In addition,high levels of MED8 in HCC are positive correlation with poor overall survival and disease-related survival.Receiver Operating Characteristic(ROC)curve results indicate that the area under the curve(AUC)of MED8 is 0.817,indicating that MED8 may be a potential diagnostic molecular marker for HCC.Conclusion MED8 is closely related to the clinical stage and prognosis of HCC patients,and it may affect the prognosis of patients through the complement activation pathway.

关 键 词：肝细胞肝癌 MED8 C1qc 生物信息分析 预后 

分 类 号：R735.7[医药卫生—肿瘤]