基于液相色谱-质谱的视神经炎患者血清代谢组学初步分析  

Preliminary analysis of serum metabolomics in patients with optic neuritis based on liquid chromatography-mass spectrometry

作　　者：罗保斌 钱婧雨 崔世磊[2] 何小魁[1] 张晓红[1] 刘向祎[1] Luo Baobin;Qian Jingyu;Cui Shilei;He Xiaokui;Zhang Xiaohong;Liu Xiangyi(Department of Clinical Laboratory,Beijing Tongren Hospital,Capital Medical University,Beijing 100730,China;Department of Neurology,Beijing Tongren Hospital,Capital Medical University,Beijing 100730,China)

机构地区：[1]首都医科大学附属北京同仁医院检验科,北京100730 [2]首都医科大学附属北京同仁医院神经内科,北京100730

出　　处：《中华检验医学杂志》2024年第2期147-151,共5页Chinese Journal of Laboratory Medicine

基　　金：中国高校产学研创新基金(2023JQ022)。

摘　　要：目的分析视神经炎患者血清特征性代谢物。方法病例对照研究。收集2021年1月至2022年1月北京同仁医院神经内科确诊的视神经炎患者血清样本,同时收集性别、年龄匹配的健康体检者血清样本作为对照组。其中,视神经炎组9例,女性5例,男性4例,年龄(35.8±12.9)岁,对照组9例,女性5例,男性4例,年龄(32.6±8.6)岁。采用液相色谱质谱技术分析视神经炎患者、健康人群血清中的代谢物。选择主成分分析(PCA)及正交偏最小二乘法分析(OPLS-DA)的多元统计分析方法,采用OPLS-DA模型的变量投影重要性(VIP)值并结合t检验的P值筛选差异代谢物。结果鉴定出37种特征代谢物,VIP>1且P<0.05的差异代谢物共有4种,3种为磷脂类物质,分别为LysoPC(P-16∶0)、LysoPC(16∶0)、LysoPC(P-18∶0),1种为苏氨酸,4种代谢物含量均下调,4种代谢物受试者工作曲线下面积分别为0.951、0.889、0.963和0.944。结论基于代谢组学分析,视神经炎患者血清中的一些代谢物发生了变化,可为视神经炎血清标志物的筛选提供实验基础。Objective To investigate the serum metabolites and their metabolic characteristics of patients with optic neuritis.Methods Case-control study.From January 2021 to January 2022,9 serum specimens of diagnosed patients with optic neuritis were collected in Department of Neurology from Beijing Tongren Hospital and 9 healthy subjects as the control.Among them,there were 5 females and 4 males in the optic neuritis group,aged(35.8±12.9)years;there were 5 females and 4 males in the healthy control group,aged(32.6±8.6)years.Liquid chromatography-mass spectrometry was used to detect metabolites in serum of healthy control and patients with optic neuritis.The principal component analysis(PCA)and orthogonal partial least-squares discriminination analysis(OPLS-DA)were used to analyze the differential metabolites.The variable importance projection value of OPLS-DA model and the P value of t-test was applied to find the different metabolites.Results Thirty-seven metabolites were finally identified from serum samples.Four metabolites with variable important in projection(VIP)values larger than 1 and P values less than 0.05 were teased out,three metabolites,LysoPC(P-16∶0),LysoPC(16∶0),LysoPC(P-18∶0)belonge to phospholipid and one metabolite was L-Threonine,they were all down-regulated.The area under curve were 0.951,0.889,0.963 and 0.944,respectively.Conclusion Based on metabonomic analysis,some metabolites in serum have changed,which can provide basis for biomarkers screening of optic neuritis.

关 键 词：视神经炎 代谢 生物标志物 液质色谱-质谱法 

分 类 号：R774.61[医药卫生—眼科]