Unleashing the healing potential:Exploring next-generation regenerative protein nanoscaffolds for burn wound recovery  

作　　者：Liangwei Si Xiong Guo Hriday Bera Yang Chen Fangfang Xiu Peixin Liu Chunwei Zhao Yasir Faraz Abbasi Xing Tang Vito Foderà Dongmei Cun Mingshi Yang 

机构地区：[1]Wuya College of Innovation,Shenyang Pharmaceutical University,Shenyang 110016,China [2]Dr.B.C.Roy College of Pharmacy and Allied Health Sciences,Durgapur 713206,India [3]Department of Pharmaceutics,School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China [4]Department of Pharmacy,Faculty of Health and Medical Sciences,University of Copenhagen,niversitetsparken 2,DK-2100 Copenhagen O,Denmark

出　　处：《Asian Journal of Pharmaceutical Sciences》2023年第6期100-112,共13页亚洲药物制剂科学（英文）

基　　金：supported by the Liaoning Pan Deng Xue Zhe Scholar(No.XLYC2002061);the Overseas Expertise Introduction Project for Discipline Innovation(“111 Project”)(No.D20029);X.G.acknowledges the Educational Department of Liaoning Province(grant No.LJKZ0925),(Youth Project,grant No.LJKQZ2021035);the international postdoctoral exchange fellowship program(grant No.PC2021047);H.B.thanks the financial support from National Natural Science Foundation of China(grant No.82050410448);Fellowship of China Postdoctoral Science Foundation(grant No.2021MD703857);D.Cun acknowledges financial support from Ministry of Education Chunhui Program(2020);VF acknowledges VILLUM FONDEN for supporting the project via the Villum Young Investigator Grant(grant No.19175).

摘　　要：Burn injury is a serious public health problem and scientists are continuously aiming to develop promising biomimetic dressings for effective burn wound management.In this study,a greater efficacy in burn wound healing and the associated mechanisms ofα-lactalbumin(ALA)based electrospun nanofibrous scaffolds(ENs)as compared to other regenerative protein scaffolds were established.Bovine serum albumin(BSA),collagen type I(COL),lysozyme(LZM)and ALA were separately blended with poly(ε-caprolactone)(PCL)to fabricate four different composite ENs(LZM/PCL,BSA/PCL,COL/PCL and ALA/PCL ENs).The hydrophilic composite scaffolds exhibited an enhancedwettability and variablemechanical properties.The ALA/PCL ENs demonstrated higher levels of fibroblast proliferation and adhesion than the other composite ENs.As compared to PCL ENs and other composite scaffolds,the ALA/PCL ENs also promoted a better maturity of the regenerative skin tissues and showed a comparable wound healing effect to Collagen sponge^(■)on third-degree burn model.The enhanced wound healing activity of ALA/PCL ENs compared to other ENs could be attributed to their ability to promote serotonin production at wound sites.Collectively,this investigation demonstrated that ALA is a unique protein with a greater potential for burn wound healing as compared to other regenerative proteins when loaded in the nanofibrous scaffolds.

关 键 词：Regenerative proteins Α-LACTALBUMIN SEROTONIN Electrospinning Nanofibrous dressing Third-degree burn 

分 类 号：R318.08[医药卫生—生物医学工程] TQ340.64[医药卫生—基础医学] TB383.1[化学工程—化纤工业]