Promoter variations in DBR2-like affect artemisinin production in different chemotypes of Artemisia annua  

作　　者：Xingwen Wang Lan Wu Li Xiang Ranran Gao Qinggang Yin Mengyue Wang Zhaoyu Liu Liang Leng Yanyan Su Huihua Wan Tingyu Ma Shilin Chen Yuhua Shi 

机构地区：[1]Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine,Artemisinin Research Center,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China [2]Institute of Herbgenomics,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China [3]Amway(China)Botanical R&D Center,Wuxi 214115,China [4]Key Lab of Chinese Medicine Resources Conservation,State Administration of Traditional Chinese Medicine of the People’s Republic of China,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100193,China

出　　处：《Horticulture Research》2023年第9期220-232,共13页园艺研究（英文）

基　　金：supported by the Fundamental Research Funds for the Central Public Welfare Research Institutes(ZZ13-YQ-101,ZZ16-ND-10-09,ZZ16-ND-10-28);the CACMS Innovation Fund(CI2021A04107),the National Natural Science Foundation of China(U1812403-1);the National Key Research and Development Project(2019YFE0108700,China);the Fundamental Research Funds for the Central Universities(3332022051).

摘　　要：Artemisia annua is the only known plant source of the potent antimalarial artemisinin,which occurs as the low-and high-artemisinin producing(LAP and HAP)chemotypes.Nevertheless,the different mechanisms of artemisinin producing between these two chemotypes were still not fully understood.Here,we performed a comprehensive analysis of genome resequencing,metabolome,and transcriptome data to systematically compare the difference in the LAP chemotype JL and HAP chemotype HAN.Metabolites analysis revealed that 72.18%of sesquiterpenes was highly accumulated in HAN compared to JL.Integrated omics analysis found a DBR2-Like(DBR2L)gene may be involved in artemisinin biosynthesis.DBR2L was highly homologous with DBR2,belonged to ORR3 family,and had the DBR2 activity of catalyzing artemisinic aldehyde to dihydroartemisinic aldehyde.Genome resequencing and promoter cloning revealed that complicated variations existed in DBR2L promoters among different varieties of A.annua and were clustered into three variation types.The promoter activity of diverse variant types showed obvious differences.Furthermore,the core region(-625 to 0)of the DBR2L promoter was identified and candidate transcription factors involved in DBR2L regulation were screened.Thus,the result indicates that DBR2L is another key enzyme involved in artemisinin biosynthesis.The promoter variation in DBR2L affects its expression level,and thereby may result in the different yield of artemisinin in varieties of A.annua.It provides a novel insight into the mechanism of artemisinin-producing difference in LAP and HAP chemotypes of A.annua,and will assist in a high yield of artemisinin in A.annua.

关 键 词：thereby INSIGHT HOMOLOGOUS 

分 类 号：Q946.8[生物学—植物学]