功能性鱼软骨多糖制备工艺优化及食用安全性评价  

Optimization of the Preparation Process of Functional Fish Cartilage Polysaccharide and Its Safety Evaluation for Consumption

作　　者：武瑞赟 桂萌[3] 刘子宇 Tharushi S.Shinali 尚楠 WU Ruiyun;GUI Meng;LIU Ziyu;Tharushi SShinali;SHANG Nan(Research Institute of Agricultural Products Processing,Chinese Academy of Agricultural Sciences,Beijing 100193,China;College of Engineering,China Agricultural University,Beijing 100083,China;Beijing Fisheries Research Institute,Beijing 100068,China;Community Health Service Center of Beijing Language and Culture University,Beijing 100083,China)

机构地区：[1]中国农业科学院农产品加工研究所,北京100193 [2]中国农业大学工学院,北京100083 [3]北京市农林科学院水产科学院研究所,北京100068 [4]北京语言大学社区卫生服务中心,北京100083

出　　处：《肉类研究》2023年第12期7-15,共9页Meat Research

基　　金：现代农业产业技术体系北京市渔业创新团队项目(BCIC07-2023-13)。

摘　　要：低分子质量硫酸软骨多糖(chondroitin sulfate,CS)具有比高分子质量CS更好的生物利用度和生物活性。为获得抗结直肠癌活性较高的低分子质量CS,使用杂交鲟(Acipenser schrenckii×Huso dauricus)SC(sturgeon chondroitin sulfate,SCS)为原料,以对结直肠癌细胞HT-29细胞增殖抑制率为指标,采用酶促解聚法结合响应面法确定制备具有抗结直肠癌活性低分子质量SCS的最佳酶解条件为酶添加量0.103 IU/mg、底物质量浓度1.005 mg/mL、酶解时间87 min。在此基础上,利用超滤法制备低分子质量高活性的组分SCS-F2;为探讨SCS-F2的食用安全性,利用动物模型评价SCS-F2的急性毒性,结果表明,SCS-F2对小鼠体质量、饮食及血液学指标及机体脏器组织无不良影响,且可减低结肠组织的异常隐窝灶,说明SCS-F2对结直肠癌变具有一定的抑制和预防作用且满足食品保健品安全要求。因此,SCS-F2安全无毒,具有抗结直肠癌活性。Low-molecular-mass chondroitin sulfate(CS)have attracted wide attention due to their better bioavailability and biological activity than highmolecular-mass CS.To obtain low-molecular-mass sulfate chondropolysaccharides with higher anti-colorectal cancer activity,sturgeon chondroitin sulfate(SCS)from the hybrid sturgeon Acipenser schrenckii×Huso dauricus was enzymatically depolymerized.Response surface methodology was used to optimize the enzymatic process based on inhibitory activity against the proliferation of HT-29 cells.The optimal enzymatic conditions were determined as 0.103 IU/mg,1.005 mg/mL and 87 min for enzyme dosage,substrate concentration,and enzymatic digestion time,respectively.Furthermore,SCS-F2 with low molecular mass and high anticancer activity was prepared by ultrafiltration.In order to explore its safety for consumption,the acute toxicity of SCS-F2 was evaluated by using an animal model.The experimental results showed that SCS-F2 has no adverse effects on body mass,food intake,blood biochemical indexes or visceral tissues while reducing the number of aberrant colonic crypts,indicating that SCS-F2 has inhibitory and preventive effects on colorectal cancer and meets the safety requirements of food and health products.Therefore,SCS-F2 is safe and non-toxic,and has anti-colorectal cancer activity.

关 键 词：低分子质量硫酸软骨多糖 制备 结直肠癌 安全性 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]