Unveiling plant defense arsenal: metabolic strategies in Brassica oleracea during black rot disease  

作　　者：Carmen Vega-Álvarez Pilar Soengas Thomas Roitsch Rosaura Abilleira Pablo Velasco Marta Francisco 

机构地区：[1]Group of Genetics,Breeding and Biochemistry of Brassicas,Misión Biológica de Galicia(CSIC),ES-36143,Pontevedra,Spain [2]Department of Plant and Environmental Sciences,Copenhagen Plant Science Centre,University of Copenhagen,DK-2630,Taastrup,Denmark

出　　处：《Horticulture Research》2023年第11期120-130,共11页园艺研究（英文）

基　　金：supported by the research projects PID2021-126472OB-I00 and RTI2018-094650-J-100 of the Ministry of Science and Innovation,the Government of Spain.Carmen VegaÁlvarez acknowledges a PFI fellowship from the Spanish Ministry of Science and Innovation;Marta Francisco acknowledges the Ramón y Cajal Research Program(RYC2019-027834-I)through the MCIN/AEI/10.13039/501100011033 and“ESF Investing in your future”.Thomas Roitschwould like to acknowledge funding by the Ministry of Education,Youth and Sports of Czech Republic within the National Sustainability Programme I(NPU I),grant number LO1415.

摘　　要：Alterations in plant metabolism play a key role in the complex plant-pathogen interactions.However,there is still a lack of knowledge about the connection between changes in primary and specialized metabolism and the plant defense against diseases that impact crops.Thus,we aim to study the metabolic reprograming in Brassica oleracea plants upon infection by Xanthomonas campestris pv.campestris(Xcc).To accomplish this,we utilized a combination of untargeted and targeted metabolomics,through UPLC-Q-TOF-MS/MS and 1H-NMR,in two crop lines differing in resistance thatwere evaluated at two-and four-week intervals following inoculation(T1 and T2,respectively).Besides,to depict the physiological status of the plant during infection,enzymatic activities related to the carbohydrate pathway and oxidative stress were studied.Our results revealed different temporal dynamics in the responses of the susceptible vs.resistant crops lines.Resistant B.oleracea line suppresses carbohydrate metabolism contributing to limit nutrient supplies to the bacterium and prioritizes the induction of defensive compounds such as indolic glucosinolates,salicylic acid,phenylpropanoids and phytoalexins precursors at early infection stages.In contrast,the susceptible line invests in carbohydratemetabolism,including enzymatic activities related to the hexoses turnover,and activates defense signaling related to reactive oxygen species.Thus,each line triggers a different metabolic strategy that will affect how the plant overcomes the disease in terms of resistance and growth.This work provides first insights of a fine-tuned metabolic regulation during Xcc infection in B.oleracea that will contribute to develop new strategies for plant disease management.

关 键 词：METABOLIC METABOLISM BLACK 

分 类 号：S635[农业科学—蔬菜学]