基于生物信息学分析NUSAP1在胰腺癌中的表达及临床预后分析  

Analysis of NUSAP1 expression and clinical prognosis in pancreatic cancer based on bioinformatics

作　　者：王洁[1] 王圣泳 阳文芳 程若溪 吴青[1] 彭鹏[1] 黄杰安[1] 覃蒙斌[1] WANG Jie;WANG Sheng-yong;YANG Wen-fang;CHENG Ruo-xi;WU Qing;PENG Peng;HUANG Jie-an;QIN Meng-bin(Department of Gastroenterology,the Second Affiliated Hospital of Guangxi Medical University,Nanning 530007,China;Department of Neurosurgery,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学第二附属医院消化内科,530007 [2]广西医科大学第一附属医院神经外科,530021

出　　处：《现代消化及介入诊疗》2023年第12期1514-1518,共5页Modern Interventional Diagnosis and Treatment in Gastroenterology

基　　金：广西医科大学青年科学基金资助项目(GXMUYSF202347);广西自然科学基金区域高发疾病研究联合专项资助(2023GXNSFAA026455);广西卫生健康委员会自筹经费科研课题(Z20210625)。

摘　　要：目的 研究核仁纺锤体相关蛋白1 (Nucleolar and spindle associated protein 1,NUSAP1)在胰腺癌组织中的表达及临床预后分析。方法 利用GEPIA数据库检索NUSAP1基因在胰腺癌组织和正常组织中的表达水平,TCGA数据库及组织芯片研究及验证NUSAP1在胰腺癌的临床预后分析。结果 GEPIA数据库分析结果显示胰腺癌组织中NUSAP1的表达高于正常对照组(P<0.05)。TCGA数据库结果显示,高表达NUSAP1的胰腺癌患者生存时间较低表达NUSAP1的患者更短(HR=2.02,P<0.05)。在病理分级G3-G4组、残余瘤R1和R2组、胰腺癌治疗进展组中NUSAP1的表达均分别高于病理分级G1-G2组、残余瘤R0组、治疗缓解组(P<0.05)。在死亡组中,NUSAP1的表达高于存活组(P<0.05)。组织芯片免疫组化结果显示,与癌旁组织相比,NUSAP1在胰腺癌中呈高表达(χ^(2)=44.10,P<0.001),NUSAP1高表达的患者生存时间更短,预后越差。单因素回归分析结果显示NUSAP1的表达量、性别、病理分级、肿瘤大小、T分期、N分期、TNM分期是影响胰腺癌患者总生存期的危险因素(P<0.05)。多因素回归分析结果显示NUSAP1的表达量、TNM分期是影响总生存期的独立危险因素(P<0.05)。结论 NUSAP1在胰腺癌组织中高表达,与胰腺癌患者不良预后相关。Objective To investigate the expression and prognosis of Nucleolar and spindle associated protein 1(NUSAP1) in pancreatic cancer.Methods The expression level of NUSAP1 gene in pancreatic cancer tissue and normal tissue was retrieved and downloaded using GEPIA database,and the clinical prognosis analysis of NUSAP1 in pancreatic cancer was researched and verified by TCGA database and tissue chip.Results The results of GEPIA database analysis showed that the expression of NUSAP1 in pancreatic cancer tissues was higher than that in normal control group(P< 0.05).TCGA database results showed that pancreatic cancer patients with high expression of NUSAP1 had shorter survival time than patients with low expression of NUSAP1(HR=2.02,P<0.05).The expression of NUSAP1 in G3-G4 group,residual tumor R1 and R2 group and pancreatic cancer progression group was higher than that in G1-G2 group,residual tumor R0 group and treatment remission group,respectively(P<0.05).In the death group,the expression of NUSAP1 was higher than that in the survival group(P<0.05).Immunohistochemistry results of tissue chip showed that NUSAP1 was highly expressed in pancreatic cancer compared with adjacent tissues( χ^(2)=44.10,P<0.001).Patients with high NUSAP1 expression had shorter survival time and worse prognosis.Univariate regression analysis showed that NUSAP1 expression level,sex,pathological grade,tumor size,T stage,N stage and TNM stage were risk factors affecting the overall survival of patients with pancreatic cancer(P<0.05).Multivariate regression analysis showed that the expression level of NUSAP1 and TNM stage were independent risk factors for overall survival(P<0.05).Conclusion NUSAP1 is highly expressed in pancreatic cancer tissues and is associated with poor prognosis in patients with pancreatic cancer.

关 键 词：生物信息学 核仁纺锤体相关蛋白1 胰腺癌 临床预后 

分 类 号：R576[医药卫生—消化系统]