巨噬细胞膜仿生纳米铁颗粒制备及多形性胶质母细胞瘤MRI成像的初步实验研究  

Experimental Study of Nanoparticles with Biomimetic Macrophage Membrane for MRI Imaging of Glioblastoma

作　　者：余宏微 陈静文 贺美娟 范旭辉 王毅晖[1] 王悍[1] YU Hong-wei;CHEN Jing-wen;HE Mei-juan;FAN Xu-hui;WANG Yi-hui;WANG Han△(Department of Radiology,Shanghai General Hospital,Shanghai Jiao Tong University School of medicine,Shanghai,200080,China)

机构地区：[1]上海交通大学医学院附属第一人民医院放射科,上海200080

出　　处：《现代生物医学进展》2024年第1期6-11,共6页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(82271969);上海市教委重大项目(202101070002E00085);上海市科学技术委员会项目(19411951403)。

摘　　要：目的:探讨巨噬细胞膜仿生的纳米铁颗粒(Fe3O4 NCs@MM)对多形性胶质母细胞瘤MRI成像的研究。方法:制备巨噬细胞膜仿生的纳米铁颗粒Fe3O4 NCs@MM,利用动态光散射(Dynamic Light Scattering,DLS)和透射电子显微镜(Transmission Electron Microscope,TEM)对其水合动力学粒径、表面电势和形态进行表征。采用SDS-聚丙烯酰胺凝胶电泳(sodium dodecyl sulphate-polyacrylamide gel electrophoresis,SDS-PAGE)评价巨噬细胞膜的完整包覆;紫外可见光谱测定巨噬细胞膜仿生的纳米铁颗粒抗蛋白吸附能力。通过MRI成像系统,分析了含不同浓度的Fe元素(0.1-1.6 mM)的Fe3O4 NCs@MM在GSH存在或不存在时的T1弛豫效应。采用细胞增殖-毒性实验(Cell Counting Kit-8,CCK-8),测定巨噬细胞膜仿生纳米铁颗粒处理肿瘤细胞24 h后的细胞活性。尾静脉注射巨噬细胞膜仿生纳米铁颗粒至原位胶质母细胞瘤模型中,观察成像效果。结果:巨噬细胞膜仿生的纳米铁颗粒Fe3O4 NCs@MM的水合动力学粒径和表面电势分别为286.5±7.6 nm和-20.7±3.5 mV,且在水溶液中分布均匀,具有较好的单分散性。包覆巨噬细胞膜的纳米铁颗粒具备抗蛋白吸附的能力。MRI成像显示,制备的巨噬细胞膜仿生的纳米铁颗粒Fe3O4 NCs@MM为GSH响应型MRI对比剂,具有较好的T1-加权磁共振成像效果,在尾静脉注射巨噬细胞膜的纳米铁颗粒0.5 h后,肿瘤部位的信号可见增强。结论:巨噬细胞膜仿生的纳米铁颗粒Fe3O4 NCs@MM可实现多形性胶质母细胞瘤的MRI成像。Objective:To investigate the MRI imaging of glioblastoma multiforme with nanoparticles biomimetic to macrophage cell membrane(Fe3O4 NCs@MM).Methods:The nanoparticles with macrophage cell membrane(Fe3O4 NCs@MM)were prepared and characterized by Dynamic Light Scattering(DLS)and Transmission Electron Microscope(TEM).Sodium dodecyl sulphate-polyacrylamide gel electrophoresis(SDS-PAGE)was used to evaluate the complete coating of extracted macrophage cell membranes.The anti-protein adsorption ability of nanoparticles with macrophage cell membrane was determined by UV-VIS spectroscopy.The T1 relaxation effects of Fe3O4NCs@MM with Fe at different concentrations(0.1-1.6 mM)in the presence or absence of GSH were analyzed by MRI.Cell Counting Kit-8(CCK-8)was used to determine the cell activity of tumor cells treated with macrophage cell membrane bionic nanoparticles for 24 h.Glioblastoma-bearing mice were injected with macrophage cell membrane biomimetic nanoparticles and scanned by MRI.Results:The hydration kinetic particle size and surface potential of macrophage cell membrane biomimetic nanoparticle(Fe3O4 NCs@MM)were 286.5±7.6 nm and-20.7±3.5 mV respectively,with good monodispersion.Nanoparticles coated with macrophage cell membrane can successfully resist protein adsorption.MRI imaging showed that the prepared macrophage membrane bionic nanoparticles(Fe3O4 NCs@MM)were considered as GSH-responsive MRI contrast agents for T1-weighted MRI imaging.The enhanced signal at the tumor site can be observed at 0.5 h post-injection of Fe3O4 [e-mail]:MRI imaging of glioblastoma multiforme can be achieved by biomimetic nanoparticles of macrophage cell membrane(Fe3O4 NCs@MM).

关 键 词：仿生纳米颗粒 MRI成像 多形性胶质母细胞瘤 

分 类 号：R-33[医药卫生] R445R739.4