ERF5.1 modulates carotenoid accumulation by interacting with CCD4.1 in Lycium  

作　　者：Jianhua Zhao Yuhui Xu Haoxia Li Xinlei Zhu Yue Yin Xiyan Zhang Xiaoya Qin Jun Zhou Linyuan Duan Xiaojie Liang Ting Huang Bo Zhang Ru Wan Zhigang Shi Youlong Cao Wei An 

机构地区：[1]National Wolfberry Engineering Research Center/Wolfberry Science Research Institute,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan,750002,China [2]Institute of Forestry and Grassland Ecology,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan,750002,China [3]College of Biological Science&Engineering,North Minzu University,Yinchuan 750021,China

出　　处：《Horticulture Research》2023年第12期190-203,共14页园艺研究（英文）

基　　金：This work was sponsored by the National Natural Science Foundation of China(No.32060359);the Key Research&Development Program of Ningxia Hui Autonomous Region(No.2021BEF02002,2022BBF01001);the Innovative Research Group Project of Ningxia Hui Autonomous Region(No.2021AAC01001);the Innovation Team for Genetic Improvement of Economic Forests(No.2022QCXTD04).

摘　　要：Carotenoids are important natural pigments and have medical and health functions for humans.Carotenoid cleavage dioxygenase 4(CCD4)and ethylene responsive factor(ERF)participate in carotenoid metabolism,but their roles in Lycium have not been discovered.Here,we annotated LbCCDs from the Lycium reference genome and found that LbCCD4.1 expression was significantly correlated with the carotenoid metabolites during Lycium five fruit developmental stages.Over-expression of LbCCD4.1 in NQ’s leaves resulted in a series of significantly lower contents of carotenoid metabolites,includingβ-carotene andβ-cryptoxanthin.Moreover,LbERF5.1,a transcription factor belonging to the ERF family that was located in the nucleus,was isolated.Significant reductions in the carotenoids,especially lutein,violaxanthin and their derivatives,were observed in over-expressing ERF5.1 transgenic NQ’s leaves.Over-expression or virus-induced gene silencing of LbERF5.1 in NQ’s leaves induced a consistent up-or down-expression,respectively,of LbCCD4.1.Furthermore,yeast one-hybrid and dual-luciferase reporter assays showed that ERF5.1 interacted with the promoter of CCD4.1 to increase its expression,and LbERF5.1 could bind to any one of the three predicted binding sites in the promoter of LbCCD4.1.A transcriptome analysis of LbERF5.1 and LbCCD4.1 over-expressed lines showed similar global transcript expression,and geranylgeranyl diphosphate synthase,phytoene synthase,lycopeneδ-cyclase cytochrome,cytochrome P450-type monooxygenase 97A,cytochrome P450-type monooxygenase 97C,and zeaxanthin epoxidase in the carotenoid biosynthesis pathway were differentially expressed.In summary,we uncovered a novel molecular mechanism of carotenoid accumulation that involved an interaction between ERF5.1 and CCD4.1,which may be used to enhance carotenoid in Lycium.

关 键 词：METABOLISM EXPRESSING LYCIUM 

分 类 号：S641.2[农业科学—蔬菜学]