异源双根缓解咖啡连作障碍及其机制初探  

Heterologous Double-root Alleviated Coffee Continuous Cropping Obstacle and the Mechanism

作　　者：孙燕[1,2] 张昂 赵青云 董云萍[1,2] 龙宇宙 林兴军[1,2] SUN Yan;ZHANG Ang;ZHAO Qingyun;DONG Yunping;LONG Yuzhou;LIN Xingjun(Spice and Beverage Research Institute,Chinese Academy of Tropical Agricultural Sciences/Innovation Platform for Academi-cian Zhang Fusuo of Hainan Province/Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops,Ministry of Agriculture and Rural Affairs/Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops,Wanning,Hainan 571533,China;Yan Lin Expert Workstation of Yunnan Province,Baoshan,Yunnan 678000,China)

机构地区：[1]中国热带农业科学院香料饮料研究所/海南省张福锁院士创新平台/农业农村部香辛饮料作物遗传资源利用重点实验室/海南省热带香辛饮料作物遗传改良与品质调控重点实验室,海南万宁571533 [2]云南省闫林专家工作站,云南保山678000

出　　处：《热带作物学报》2024年第2期323-329,共7页Chinese Journal of Tropical Crops

基　　金：海南省自然科学基金面上项目(No.321MS093,No.321MS0804);国家自然科学基金青年基金项目(No.32101847)。

摘　　要：为明确异源双根是否能缓解咖啡连作障碍及其可能机制,本研究以大粒种咖啡1号(Caffealiberica,No.1)和中粒种咖啡热研1号(Caffea canephora, Reyan No. 1)靠接形成大粒种-中粒种异源双根咖啡,通过咖啡连作障碍园土壤培养幼苗,评价植株苗木质量,明确异源双根对咖啡连作障碍的缓解作用。通过双根无分隔、尼龙网分隔、双盆分隔等不同分根方式试验,明确异源双根缓解咖啡连作障碍机制,并探讨双根间在缓解连作障碍上的差异及协同作用。结果表明:较连作障碍咖啡园非根际土,异源双根咖啡或同源双根咖啡经根际土培养后苗木质量指数、总的植株生物量均显著下降,连作逆境抑制咖啡生物量积累,使苗木质量变差;非根际土培养后,异源双根咖啡、同源双根咖啡的苗木质量指数和总的植株生物量差异均不显著;而根际土培养后,异源双根咖啡较同源双根咖啡的苗木质量指数、大粒种根系生物量、中粒种根系生物量及总的植株生物量均显著提高。因此,连作逆境虽抑制咖啡生长,但对同源双根咖啡影响更大,异源双根通过其大粒种根系缓解连作逆境咖啡生长障碍。为进一步明确异源双根尤其是大粒种根系缓解咖啡连作障碍的可能机制,以不同分根方式培养异源双根咖啡,发现较无分隔、双盆分隔处理,连作逆境下双根尼龙网分隔后植株苗木质量佳,并且尼龙网分隔后植株的中粒种根系长势最好,说明大粒种根系化感物质促进中粒种根系生长。双根无分隔、双盆分隔后植株的苗木质量差异不显著,无分隔较双盆分隔后植株的大粒种根系长势差,即双根系在能够彼此接触的自然生长状态下,中粒种根系抑制大粒种根系生长,但这种抑制作用与中粒种根际化感物质无关,尼龙网分隔、双盆分隔后大粒种根系长势差异不显著,说明大粒种根际有无中粒种根际化感物质,对大粒种根系�In order to clarify the alleviating effect of heterologous double-root on coffee continuous cropping obstacle,the soil of the continuous cropping obstacle garden of coffee was used to cultivate Caffea liberica,No.1 and C.can-ephora,Reyan No.1 heterologous double-root grafting coffee seedling.The mechanism of heterologous double-root alleviating continuous cropping obstacle was clarified through different root separation ways,and the differences and synergies of heterologous double-root in alleviating continuous cropping obstacle were discussed. Compared with non-rhizosphere soil, the seedling quality index and plant biomass of heterologous double-root coffee or homologous double-root coffee decreased significantly after culturing in rhizosphere soil. After non-rhizosphere soil culture, there were no significant differences in the seedling quality index or plant biomass between heterologous double-root coffee and homologous double-root coffee. The seedling quality index, root biomass of C. liberica and C. canephora, and total plant biomass of heterologous double-root coffee were significantly higher than those of homogenous double-root coffee after rhizosphere soil culture. Continuous cropping obstacle inhibited the growth of coffee, but had more effect on ho-mologous double-root coffee. Heterogenous double-root coffee alleviated the growth obstacle of continuous-cropping coffee by C. liberica roots. The seedlings quality of the heterologous double-root coffee separated root by nylon net was better than that of root undivided or separated root by double pot under continuous cropping, and the root growth of C. canephora was the best in nylon net separation. Root allelochemicals of C. liberica promoted the root growth of C. canephora. There was no significant difference in the seedlings quality of heterologous double-root coffee between root undivided and separated root by double pot, and the C. liberica root growth of root undivided was worse than that of double pot separation. The root growth of C. canephora inhib

关 键 词：咖啡 连作障碍 异源双根 分根培养 苗木质量 

分 类 号：S571.2[农业科学—作物学]