Notch4 mRNA在口咽鳞癌组织中表达变化及其敲降癌细胞株增殖迁移侵袭能力观察  

Notch4 mRNA expression in oropharyngeal squamous carcinoma tissues and its effects on cell proliferation,migration,and invasion

作　　者：薛绪亭 张宇良 郑希望 董振[1,2] 刘红亮 张春明[1,2] XUE Xuting;ZHANG Yuliang;ZHENG Xiwang;DONG Zhen;LIU Hongliang;ZHANG Chunming(Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer,First Hospital of Shanxi Medical University,Taiyuan 030001,China)

机构地区：[1]山西医科大学第一医院耳鼻咽喉头颈肿瘤山西省重点实验室,太原030001 [2]山西医科大学第一医院耳鼻咽喉头颈外科

出　　处：《山东医药》2024年第3期1-5,共5页Shandong Medical Journal

基　　金：山西省基础研究计划自由探索类青年科学研究项目(202103021223430;20210302124704)。

摘　　要：目的 观察Notch4 mRNA在口咽鳞癌组织和细胞株中的表达变化及敲降Notch4 mRNA表达的口咽鳞癌细胞株增殖、迁移和侵袭能力。方法 收集12对口咽鳞癌组织与癌旁组织,采用RT-q PCR法检测癌组织、癌旁组织中Notch4 mRNA。培养口咽鳞癌细胞株Detroit 562、WSU-HN-30及对照细胞株Ha CaT、2BS,检测细胞株中Notch4 mRNA。将人口咽鳞癌细胞株Detroit 562和WSU-HN-30随机分为si-NC组、si-Notch4-1组和si-Notch4-2组,分别转染si-NC双链核苷酸、si-Notch4-1双链核苷酸、si-Notch4-2双链核苷酸,检测各组细胞株中Notch4 mRNA,使用RTCA法观察各组细胞株的增殖能力(CI值),通过Transwell实验观察各组细胞株的侵袭和迁移能力。结果 与癌旁组织相比,口咽鳞癌组织中Notch4 mRNA相对表达量高(P<0.01);在对照细胞株Ha CaT和2BS中Notch4 mRNA相对表达量差异无统计学意义;与对照细胞株Ha CaT相比,口咽鳞癌细胞株Detroit 562、WSU-HN-30中Notch4 mRNA相对表达量高(P均<0.001)。在口咽鳞癌细胞株Detroit 562、WSU-HN-30中,与si-NC组相比,si-Notch4-1和si-Notch4-2组的细胞株增殖、迁移和侵袭能力降低(P均<0.01)。结论 Notch4 mRNA在口咽鳞癌组织和细胞株中均高表达,敲降Notch4 mRNA表达的口咽鳞癌细胞株增殖、迁移和侵袭能力降低。Objective To observe the expression changes of Notch4 mRNA in tissues and cells of oropharyngeal squamous cell carcinoma,and to explore the effect of knocking down Notch4 mRNA expression on the proliferation,migration,and invasion abilities of oropharyngeal squamous cell carcinoma cells.Methods We collected 12 pairs of oropharyngeal squamous cell carcinoma tissues and adjacent tissues,and detected Notch4 mRNA expression levels in the cancer tissues and adjacent tissues by RT-qPCR.We cultivated oropharyngeal squamous cell carcinoma cell lines Detroit 562 and WSU-HN-30,and control cells HaCaT,2BS to detect Notch4 mRNA.The cell lines Detroit 562 and WSU-HN-30 were randomly divided into the si-NC group(transfected with si-NC double stranded nucleotide),si-Notch4-1 group(transfected with si-Notch4-1 double stranded nucleotide),and si-Notch4-2 group(transfected with si-Notch4-2 double stranded nucleotide).Notch4 mRNA was detected in cells of each group,and the proliferation ability(CI value) of cells in each group was observed using RTCA method.The invasion and migration abilities of cells in each group were observed by Transwell experiment.Results Compared with the adjacent tissues,the relative expression level of Notch4 mRNA in oropharyngeal squamous cell carcinoma tissues was higher(P<0.01).There was no difference in the relative expression level of Notch4 mRNA between HaCaT and 2BS cells in the control group.Compared with HaCaT cells in the control group,the relative expression levels of Notch4 mRNA were higher in the Detroit 562 and WSU-HN-30 cells(both P<0.01).In the Detroit 562 and WSU-HN-30 cells,compared with the si-NC group,the cell proliferation,migration,and invasion abilities of the si-Notch4-1 and si-Notch4-2 groups decreased(all P<0.001).Conclusion Notch4 was highly expressed in oropharyngeal squamous carcinoma tissues and cells,and knockdown of Notch4 expression significantly inhibited the proliferation,migration and invasion of oropharyngeal squamous carcinoma cells.

关 键 词：Notch家族跨膜受体 口咽鳞状细胞癌 细胞增殖 细胞迁移 细胞侵袭 

分 类 号：R739.63[医药卫生—肿瘤]