谷丙转氨酶2在食管鳞癌组织中表达变化及对癌细胞增殖迁移侵袭能力影响  

Expression of GPT2 in ESCC tissues and its effects on cell proliferation,migration and invasion

作　　者：刘龙飞 郭霞梅 罗超 张莉[1] 王其龙[1] LIU Longfei;GUO Xiamei;LUO Chao;ZHANG Li;WANG Qilong(Department of Central Laboratory,Huai'an No.1 People's Hospital Affiliated to Nanjing Medical University,Huai'an 223300,China)

机构地区：[1]南京医科大学附属淮安第一医院中心实验室,江苏淮安223300

出　　处：《山东医药》2024年第3期6-11,共6页Shandong Medical Journal

基　　金：江苏省双创博士基金项目(JSSCBS20211626);江苏省自然科学基金面上项目(BK20211111)。

摘　　要：目的 观察谷丙转氨酶2(GPT2)在食管鳞状细胞癌(ESCC)组织中的表达变化,分析GPT2与患者临床病理特征、预后的关系以及对ESCC细胞增殖、迁移和侵袭的影响,以探讨其与ESCC进展的关系。方法 选取80例ESCC患者癌组织及癌旁正常组织,将其中3例份样本通过转录组测序筛选出差异表达基因GPT2,并在TCGA数据库中进行验证。采用Western blotting及免疫组化法检测ESCC组织中GPT2蛋白,分析癌组织中GPT2表达与患者临床病理特征的关系。采用多因素Cox比例风险分析影响ESCC患者生存的风险因素,Kaplan-Meier法对GPT2高、低表达的ESCC患者进行生存分析。构建p IRES2/GPT2(GPT2过表达)质粒,然后将空载质粒(p IRES2)及p IRES2/GPT2转染至人食管鳞癌细胞系KYSE150、KYSE30(转染的细胞分别命名为p IRES2、p IRES2/GPT2组),培养48 h后,采用CCK-8及Transwell实验观察ESCC细胞增殖、迁移和侵袭能力。结果 转录组测序结果及基于TCGA数据库数据分析结果显示,与癌旁正常组织相比,ESCC组织中GPT2表达水平降低(P均<0.05)。Western blotting及免疫组化结果显示,ESCC组织中GPT2蛋白水平下调(P均<0.05)。ESCC癌组织中GPT2表达与淋巴结转移相关(P<0.05)。淋巴结转移、高病理分期和低GPT2表达是ESCC患者的生存风险因素(P均<0.05)。GPT2低表达ESCC患者生存率较高表达患者低(P<0.05)。过表达GPT2的ESCC细胞增殖、迁移和侵袭能力均降低(P均<0.05)。结论 ESCC组织中GPT2表达下调,低表达GPT2的患者预后较差。过表达GPT2可抑制ESCC细胞增殖、迁移、侵袭。Objective To observe the expression of glutamic pyruvate transaminase 2(GPT2) in the esophageal squamous cell carcinoma(ESCC) tissues,and to analyze the relationships between GPT2 and the clinicopathological features and prognosis of patients,as well as its effects on the proliferation,migration and invasion of ESCC cells in order to explore its relationship with progression of ESCC.Methods Eighty cases of ESCC tumor tissues and adjacent normal tissues were selected,and 3 of them were screened for differentially expressed genes by transcrip tome sequencing.The lowly expressed gene GPT2 was identified as the research object,and its expression in ESCC was verified in TCGA database.Western blotting and immunohistochemistry were used to detect the protein levels of GPT2 in ESCC tissues,respectively.Multivariate Cox survival risk analysis was used to determine the risk factors affecting the survival of ESCC patients,and the relationship between GPT2 expression and clinicopathological characteristics of patients was explored.Kaplan-Meier survival analysis was used to analyze the survival of patients with high or low GPT2 expression.The pIRES2/GPT2(GPT2 overexpression) plasmid was constructed,and then the empty plasmid(pIRES2) and pIRES2/GPT2 were transfected into human esophageal squamous cell lines KYSE150 and KYSE30(transfected cells were named pIRES2 and pIRES2/GPT2 groups,respectively).After 48 h of culture,the proliferation,migration and invasion abilities of ESCC cells were observed by CCK-8,and Transwell assay.Results Transcriptome sequencing results showed that the expression level of GPT2 decreased in ESCC tissues in comparison with that of the adjacent normal tissues(P<0.05).Western blotting and immunohistochemistry confirmed that GPT2 protein levels were markedly down-regulated in the ESCC tissues(both P<0.05).Multivariate Cox survival risk analysis showed that lymph node metastasis,pathological stage and low expression of GPT2were the survival risk factors of ESCC patients(all P<0.05),and GPT2 expression wa

关 键 词：谷丙转氨酶2 食管鳞状细胞癌 细胞恶性表型 细胞增殖 细胞迁移 细胞侵袭 

分 类 号：R735.1[医药卫生—肿瘤]