党参炔苷调节AKT/GSK-3β/snail信号通路对胃癌细胞增殖、凋亡和上皮间质转化的影响  

Impacts of lobetyolin on the proliferation,apoptosis and epithelial-mesenchymal transformation of gastric cancer cells by regulating the AKT/GSK-3β/snail signaling pathway

作　　者：廖美荣 陈才伟 符晶[1] 苏珊珊 周菲 LIAO Meirong;CHEN Caiwei;FU Jing(Department of Gastroenterology,the First Affiliated Hospital of Hainan Medical University,Hainan,Haikou 570100,China;不详)

机构地区：[1]海南医学院第一附属医院消化内科,海口市570100 [2]海南医学院第一附属医院健康管理中心,海口市570100

出　　处：《河北医药》2024年第4期485-489,495,共6页Hebei Medical Journal

基　　金：海南省卫生健康行业科研项目(编号:22A200047)。

摘　　要：目的探究党参炔苷(LOB)调节蛋白激酶B(AKT)/糖原合成酶激酶3β(GSK-3β)/Snail信号通路对胃癌(GC)细胞增殖、凋亡和上皮间质转化(EMT)的影响。方法将GC细胞株SGC7901随机分为对照组(Control组)、低浓度LOB组(LOB-L组,10μmol/L LOB)、中浓度LOB组(LOB-M组,20μmol/L LOB)、高浓度LOB组(LOB-H组,40μmol/L LOB)和高浓度LOB+AKT激活剂SC79组(LOB-H+SC79组,40μmol/L LOB+20μmol/L SC79)。CCK-8法检测5组细胞增殖能力。划痕实验检测细胞迁移能力。Transwell实验检测细胞侵袭能力。流式细胞术检测细胞凋亡。实时荧光定量PCR(RT-qPCR)法测定5组细胞AKT、GSK-3β、snail1 mRNA的表达。Western Blot检测5组细胞AKT/GSK-3β/snail信号通路和凋亡、EMT过程相关蛋白表达。结果与Control组比较,LOB-M组、LOB-H组SGC7901细胞OD450值(48 h、72 h)、细胞划痕愈合率、细胞侵袭数目、AKT、GSK-3β、snail1 mRNA表达和AKT、GSK-3β磷酸化水平、snail1、Bcl-2、N-cadherin、Vimentin蛋白表达显著下降(P<0.05),细胞凋亡率、Bax、E-cadherin蛋白表达显著升高(P<0.05)。SC79减弱了LOB对GC细胞增殖和EMT的抑制作用,抑制了细胞凋亡。结论LOB可能通过下调AKT/GSK-3β/snail信号通路抑制GC细胞增殖和EMT过程,促进细胞凋亡。Objective To investigate the impacts of lobetyolin(LOB)on the proliferation,apoptosis and epithelial-mesenchymal transformation(EMT)of gastric cancer (GC)cells by regulating the protein kinase B(AKT)/glycogen synthase kinase 3β(GSK-3β)/Snail signaling pathway.Methods The GC cell line SGC7901 was induced with blank control,low-dose LOB(10μmol/L LOB),medium-dose LOB(20μmol/L LOB),high-dose LOB(40μmol/L LOB)and high-dose LOB+SC79(40μmol/L LOB+AKT activator SC7920μmol/L).Cell proliferation,migration and invasion were detected by CCK-8,wound healing and Transwell assay,respectively.The mRNA levels of AKT,GSK-3β,and snail1 were detected by Reverse transcription quantitative real-time polymerase chain reaction(RT-qPCR).Protein expressions of key proteins in the AKT/GSK-3β/snail signaling pathway and those associated with apoptosis and EMT were detected by Western blot.Results The optical density at 450nm(OD450)value at 48h and 72h,wound healing rate,invasive cell number,mRNA levels of AKT,GSK-3βand snail1 and protein levels of AKT,phosphorylated GSK-3β,snail1,Bcl-2,N-cadherin and Vimentin were significantly lower in SGC7901 cells induced with medium-dose and high-dose LOB than those induced with blank control(P<0.05),while the apoptotic rate and protein expressions of Bax and E-cadherin were significantly higher (P<0.05).The treatment of SC79 weakened the inhibitory effect of LOB on the proliferation and EMT of GC cells,and inhibited cell apoptosis.Conclusion LOB may inhibit the proliferation and EMT process of GC cells and promote cell apoptosis by inhibiting the AKT/GSK-3β/snail signal pathway.

关 键 词：党参炔苷 蛋白激酶B/糖原合成酶激酶3β/Snail信号通路 胃癌 上皮间质转化 

分 类 号：R735.2[医药卫生—肿瘤]