MTAP在前列腺癌中的表达及对免疫细胞的影响初探  

The expression of MTAP in prostate cancer and its effect on immune cells

作　　者：秦梓榛 刘清源 武雨琦 苏帅 张金栋 李月 王德林[1] Qin Zizhen;Liu Qingyuan;Wu Yuqi;Su Shuai;Zhang Jindong;Li Yue;Wang Delin(Department of Urology,the First Affiliated Hospital of Chongqing Medical University;Department of Urology,South China Hospital Affiliated to Shenzhen University;Molecular Medicine and Tumor Research Center of Chongqing Medical University)

机构地区：[1]重庆医科大学附属第一医院泌尿外科,重庆400016 [2]深圳大学附属华南医院泌尿外科,深圳518055 [3]重庆医科大学分子医学与肿瘤研究中心,重庆400016

出　　处：《重庆医科大学学报》2024年第2期222-230,共9页Journal of Chongqing Medical University

摘　　要：目的:探究甲硫腺苷磷酸化酶(methylthioadenosine phosphorylase,MTAP)在前列腺癌不同临床阶段的表达差异及其对免疫细胞的影响。方法:从TCGA和GTEx数据库中获取MTAP的表达谱数据,利用生物信息学方法进行泛癌分析。免疫组化验证上述结论并探究MTAP与临床病理指标之间的关联。RT-qPCR和Western blot检测MTAP在不同前列腺癌细胞系中的表达水平,分析MTAP与肿瘤恶性程度之间的关联。生物信息学方法分析前列腺癌中MTAP与免疫细胞的关联并进行免疫组化验证。结果:在前列腺癌中,MTAP的表达在肿瘤发生早期升高后逐渐降低,与T分期和Gleason评分呈负相关(rs=-0.576,P=0.000,rs=-0.284,P=0.020)。MTAP与多种免疫细胞存在相关关系,其中MTAP与CD4+T细胞呈正相关(r=0.643,P=0.000),与NK CD56bright细胞呈负相关(r=-0.570,P=0.000)。结论:前列腺癌中,MTAP的表达呈现早期升高后逐渐下降的趋势,与T分期和Gleason评分呈负相关。MTAP对免疫细胞的浸润和细胞功能的发挥有调节作用,是一种潜在的生物学标志物和免疫治疗靶点。Objective:To explore the expression of Methylthioadenosine phosphorylase(MTAP)in different clinical stages of prostate cancer and its correlation with immune cells.Methods:The expression profile of MTAP was obtained from TCGA and GTEx databases,and pancarcinoma was analyzed by bioinformatics method.Immunohistochemistry verified the conclusions above and explored the cor⁃relation between MTAP and clinicopathological features.The expression levels of MTAP in different prostate cancer cell lines were de⁃tected by RT-qPCR and Western blot,and the correlation between MTAP and the degree of malignancy was analyzed.The correlatoin between MTAP and immune cells in prostate cancer was analyzed by bioinformatics and verified by immunohistochemistry.Results:In prostate cancer,the expression of MTAP increased at early stage and then decreased gradually,and was negatively correlated with T stage and Gleason score(rs=-0.576,P=0.000,rs=-0.284,P=0.020).MTAP was correlated with a variety of immune cells,among which MTAP was positively correlated with CD4+T cells(r=0.643,P=0.000),was significantly negatively correlated with NK CD56bright cells(r=-0.570,P=0.000).Conclusion:In prostate cancer,the expression of MTAP increased at the early stage and then decreased gradu⁃ally,which was negatively correlated with T stage and Gleason score.MTAP can regulate the infiltration and function of immune cells,and is a potential biomarker and immunotherapy target.

关 键 词：甲硫腺苷磷酸化酶 前列腺癌 生物信息学 免疫细胞 CD4 CD56 

分 类 号：R737.25[医药卫生—肿瘤]