高浓度柴油降解菌的筛选、鉴定及降解特性研究  

SCREENING,IDENTIFICATION AND DEGRADATION CHARACTERISTICS OF HIGH CONCENTRATION DIESEL DEGRADING BACTERIA

作　　者：王朝辉 曹青 李怿 李磊 王飞龙 白正伟 贾苒 Wang Zhaohui;Cao Qing;Li Yi;Li Lei;Wang Feilong;Bai Zhengwei;Jia Ran(SINOPEC Engineering Group Luoyang R&D Center of Technology,Luoyang,Henan 471003;College of Basic Medicine and Forensic Medicine,Henan University of Science and Technology)

机构地区：[1]中石化炼化工程(集团)股份有限公司洛阳技术研发中心,河南洛阳471003 [2]河南科技大学基础医学与法医学院

出　　处：《石油炼制与化工》2024年第3期118-123,共6页Petroleum Processing and Petrochemicals

基　　金：中国石油化工股份有限公司合同项目(321003);河南省高等学校重点科研项目(21B180003)。

摘　　要：从某石油化工退役场地石油烃污染土壤中筛选得到一株高效柴油降解菌株,经过形态学观察和16S rRNA基因序列分析,鉴定该菌株属于鲍特氏菌属(Bordetella),命名其为Bordetella sp.T3(简称T3)。T3适宜在中温、弱碱性的环境条件下生长,在柴油体积分数为1%、接种量(φ)为2%、温度为30℃、pH为8.0的条件下降解柴油,第7天可达89.79%的降解率。T3能够耐受柴油体积分数为10%的高浓度环境,在最适培养条件下培养7 d,其对柴油的降解率为38.57%。降解前后柴油的气相色谱对比结果表明,T3可以有效降解柴油的主要组分(C_(10)~C_(22)),具有良好的石油烃污染物修复应用潜力。A highly efficient diesel degrading strain was isolated from petroleum hydrocarbon contaminated soil at the decommission-site of a petrochemical plant.The strain was identified as Bordetella by morphological observation and 16S rRNA gene sequence analysis,named Bordetella sp.T3.T3 is suitable to grow in medium temperature and weak alkaline environment.It could degrade diesel oil under the conditions of a diesel volume fraction of 1%,an inoculation amount(φ)of 2%,a temperature of 30℃and a pH of 8.0,and the degradation rate of diesel could reach 89.79%on the 7th day.T3 could tolerate high content of 10%diesel oil(φ),and the degradation rate of diesel was 38.57%under optimal culture condition for 7 days.The results of degradation spectrogram analysis showed that T3 could effectively degrade the main components(C_(10)-C_(22))of diesel,and had a good application potential for remediation of petroleum hydrocarbon pollutants.

关 键 词：鲍特氏菌 柴油 筛选鉴定 生物降解 降解特性 

分 类 号：X172[环境科学与工程—环境科学]