H3K27me3与高级别胶质瘤生存预后的关系  

Relationship between H3K27me3 and survival prognosis of patients with high-grade gliomas

作　　者：陈智超 薛奥诺 时金昭 CHEN Zhi-chao;XUE Ao-nuo;SHI Jin-zhao(Department of Neurosurgery,Nanjing Medical University Affiliated Mingji Hospital,Nanjing 210000,China)

机构地区：[1]南京医科大学附属明基医院神经外科,南京210000

出　　处：《中国临床神经外科杂志》2023年第10期635-638,共4页Chinese Journal of Clinical Neurosurgery

基　　金：南京市医学科技发展资金(QRX21099)。

摘　　要：目的探讨组蛋白H3第27位赖氨酸三甲基化(H3K27me3)与高级别胶质瘤生存预后的关系。方法选取2015年6月至2017年9月手术切除的64例高级别胶质瘤标本,另选取颅脑损伤内减压术中切除的非肿瘤脑组织10例为对照,免疫组织化学染色法检测组织H3K27me3表达,根据染色评分分为高表达和低表达。随机选取3例非肿瘤脑组织、3例WHO分级Ⅲ级胶质瘤组织、3例WHO分级Ⅳ级胶质瘤组织,应用免疫印迹法检测H3K27me3的蛋白表达。胶质瘤病人随访截止2022年10月,记录总体生存期。结果免疫组化染色检测结果显示,高级别胶质瘤组织H3K27me3高表达率(59.37%)明显高于非肿瘤脑组织(20.00%;P<0.05)。免疫印迹法检测结果显示,WHO分级Ⅳ级胶质瘤组织H3K27me3蛋白表达水平明显高于WHO分级Ⅲ级胶质瘤组织和非肿瘤脑组织(P<0.05)。64例胶质瘤中位随访时间为62.23个月,5年生存率为37.5%。多因素Cox回归分析显示,H3K27me3高表达是高级别胶质瘤不良生存预后的独立影响因素(P<0.05)。生存曲线分析显示,H3K27me3高表达高级别胶质瘤病人中位生存期(18个月)明显低于低表达病人(33个月;P<0.05)。结论H3K27me3在高级别胶质瘤组织中高表达,与病人不良生存预后有关。Objective To investigate the relationship between expression of trimethylated histone H3 at lysine 27(H3K27me3)in glioma tissues and survival prognosis of patients with high-grade gliomas.Methods The expressions of H3K27me3 were detected in glioma tissues obtained from 64 patients with high-grade gliomas who underwent surgery from June 2015 to September 2017,and in nontumor brain tissues obtained from 10 patients with traumatic brain injury who underwent decompression using immunohistochemical staining.The protein expression of H3K27me3 was detected by western blotting in non-tumor brain tissues(n=3),WHO gradeⅢglioma tissues(n=3)and WHO gradeⅣglioma tissues(n=3).Patients with gliomas were followed up until October 2022 and their overall survival times were recorded.Results Immunohistochemical staining showed that the high expression rate of H3K27me3 in high-grade glioma tissues(59.37%)was significantly higher than that(20.00%)in non-tumor brain tissues(P<0.05).Western blotting showed that H3K27me3 protein expression level in WHO gradeⅣglioma tissues was significantly higher than those in WHO gradeⅢglioma tissues and non-tumor brain tissues(P<0.05).The median follow-up time of 64 patients with glioma was 62.23 months,and the 5-year survival rate was 37.5%.Multivariate Cox regression analysis showed that high expression of H3K27me3 was an independent risk factor for poor survival prognosis of patients with high-grade gliomas(P<0.05).Survival curve analysis showed that the median survival time(18 months)of patients with high H3K27me3 expression was significantly lower than that of patients with low H3K27ME3 expression(33 months;P<0.05).Conclusions H3K27me3 is highly expressed in high-grade gliomas,which is associated with poor survival prognosis of patients.

关 键 词：高级别胶质瘤 组蛋白H3第27位赖氨酸三甲基化(H3K27me3) 基因表达 生存预后 

分 类 号：R739.41[医药卫生—肿瘤]