鞘氨醇-1-磷酸受体激动剂芬戈莫德对黑色素瘤B16-F10增殖和迁移的影响及机制  

Effect of Sphingosine-1-phosphate Receptor Agonist Fingolimod on the Proliferation and Migration of Melanoma B16-F10 and Its Mechanism

作　　者：赵溦 马婷婷 Zhao Wei;Ma Tingting(Institute of Life Sciences,Jinzhou Medical University,Jinzhou 121000 China)

机构地区：[1]锦州医科大学生命科学研究院,辽宁锦州121000

出　　处：《锦州医科大学学报》2024年第1期13-18,共6页Journal of Jinzhou Medical University

摘　　要：目的观察芬戈莫德对黑色素瘤B16-F10细胞的增殖和自噬相关蛋白的影响。方法将含有不同浓度芬戈莫德(0、5、10、15μM)的DMEM培养基培养B16-F10细胞24 h,利用CCK-8细胞活力检测试剂盒检测各组细胞活力;划痕实验检测用药后B16-F10细胞的迁移能力;台盼蓝染色检测细胞的死活;利用免疫荧光实验和免疫印迹实验检测用药后细胞内自噬相关通路蛋白的表达情况。结果芬戈莫德可显著抑制B16-F10细胞的活力和增殖能力,并呈现剂量依赖性;芬戈莫德减弱B16-F10细胞的迁移能力并引起细胞死亡;免疫荧光和免疫印迹实验显示,与Control组相比,FTY-720组的细胞自噬通路相关蛋白LC3、Beclin1和P62的表达均出现改变(P<0.05)。结论芬戈莫德可抑制黑色素瘤B16-F10细胞的增殖并引起细胞损伤,其机制可能与芬戈莫德引起黑色素瘤B16-F10细胞的自噬有关。Objective To observe the effects of fingolimod on proliferation and autophagy-related pathway proteins in melanoma.Methods B16-F10 cells were cultured in DMEM medium containing different concentrations of fingomode(0,5,10 and 15μM)for 24 h,and cell viability was detected by CCK-8 cell viability assay kit;the migration ability of B16-F10 cells was detected by scratch assay;trypan blue staining was used to detect cell death;immunofluorescence assay and Western Blot assay were used to detect the expression of autophagy related pathway proteins.Results Fengomode significantly inhibited the viability and proliferation of B16-F10 cells in a dose-dependent manner;fengomode weakens the migration ability of B16-F10 cells and causes cell death;immunofluorescence and Western Blot showed that compared with the control group,the expressions of autophagy pathway-related proteins LC3,Beclin1 and P62 in FTY-720 group were changed(P<0.05).Conclusion Fengomode can inhibit the proliferation of melanoma B16-F10 cells and cause cell damage,and the mechanism may be related to the autophagy of B16-F10 cells induced by fengomode.

关 键 词：芬戈莫德 黑色素瘤 B16-F10 自噬 

分 类 号：R733[医药卫生—肿瘤]