CPSF6在晚期非小细胞肺癌中的表达及与预后的关系  

Expression of CPSF6 in Advanced Non-small Cell Lung Cancer and its Relationship with Prognosis

作　　者：谢明珠 朱洪斌[1] 朱楠[2] 郑炎[2] XIE Ming-zhu;ZHU Hong-bin;ZHU Nan;ZHENG Yan(Department of Respiratory Medicine,Chaohu Hospital of Anhui Medical University,Hefei 238000,Anhui,China;Department of Pathology,Chaohu Hospital of Anhui Medical University,Hefei 238000,Anhui,China)

机构地区：[1]安徽医科大学附属巢湖医院呼吸与危重症医学科,安徽巢湖238000 [2]安徽医科大学附属巢湖医院病理科,安徽巢湖238000

出　　处：《医学信息》2024年第4期95-101,共7页Journal of Medical Information

基　　金：2021年度安徽高校自然科学研究重点项目(编号:KJ2021A0336)。

摘　　要：目的探讨剪切及多聚腺苷酸化特异因子6(CPSF6)在晚期非小细胞肺癌中的表达情况及临床价值。方法利用TIMER2.0分析CPSF6在各肿瘤组织及癌旁组织中的表达差异,TCGA数据库的可视化平台GEPIA评估CPSF6在泛癌症背景下对总生存期的影响,使用GEPIA2工具比较肺癌不同病理分期(Ⅰ~Ⅳ期)中的CPSF6表达情况,在线检索TCGA数据库中肺腺癌和肺鳞癌的数据集,并进行生存和预后分析。收集2018年1月-2020年3月我院收治的74例晚期非小细胞肺癌患者肿瘤组织以及20例正常肺组织进行CPSF6免疫组化检测,Kaplan-Meier生存分析评估CPSF6表达与晚期非小细胞肺癌患者无进展生存期和总生存期的关系,单因素和多因素分析评估CPSF6对肺癌患者预后的意义。结果TIMER 2.0、TCGA数据库分析表明非小细胞肺癌患者肿瘤组织中CPSF6 mRNA水平高于癌旁组织(P<0.05),CPSF6与多种癌症(包括肺腺癌)患者的预后不良有关。CPSF6在晚期非小细胞肺癌组织表达高于正常肺组织,差异有统计学意义(P<0.05)。CPSF6表达增高与肿瘤大小、淋巴结转移情况及临床分期相关。Kaplan-Meier生存曲线显示,晚期非小细胞肺癌患者CPSF6表达增高,无进展生存期和总生存期降低。单因素及多因素分析显示,CPSF6的表达水平、淋巴结转移和临床分期均可作为晚期非小细胞肺癌患者的独立预后因子(P<0.05)。结论CPSF6在晚期非小细胞肺癌组织中表达增高,可能在其预后中发挥重要作用。Objective To investigate the expression and clinical value of cleavage and polyadenylation specific factor 6(CPSF6)in advanced non-small cell lung cancer.Methods TIMER2.0 was used to analyze the expression differences of CPSF6 in tumor tissues and adjacent tissues.The visualization platform GEPIA of TCGA database was used to evaluate the effect of CPSF6 on overall survival in the context of pan-cancer.The GEPIA2 tool was used to compare the expression of CPSF6 in different pathological stages(stagesⅠ-Ⅳ)of lung cancer.The data sets of lung adenocarcinoma and lung squamous cell carcinoma in TCGA database were retrieved online,and survival and prognosis analysis were performed.The tumor tissues of 74 patients with advanced non-small cell lung cancer admitted to our hospital from January 2018 to March 2020 and 20 lung tissues were collected for CPSF6 immunohistochemical detection.Kaplan-Meier survival analysis was used to evaluate the relationship between CPSF6 expression and progression-free survival and overall survival in patients with advanced non-small cell lung cancer.Univariate and multivariate analysis was used to evaluate the prognostic significance of CPSF6 in patients with lung cancer.Results TIMER 2.0 and TCGA database analysis showed that the level of CPSF6 mRNA in tumor tissues of patients with non-small cell lung cancer was higher than that in adjacent tissues(P<0.05).CPSF6 was associated with poor prognosis in patients with various cancers,including lung adenocarcinoma.The expression of CPSF6 in advanced non-small cell lung cancer was higher than that in lung tissue,and the difference was statistically significant(P<0.05).The increased expression of CPSF6 was correlated with tumor size,lymph node metastasis and clinical stage.Kaplan-Meier survival curve showed that CPSF6 expression was increased in patients with advanced non-small cell lung cancer,and progression-free survival and overall survival were decreased.Univariate and multivariate analysis showed that the expression level of CPSF6 protein

关 键 词：剪切及多聚腺苷酸化特异因子6 非小细胞肺癌 免疫组化 

分 类 号：R734.2[医药卫生—肿瘤]