动脉粥样硬化斑块内新生血管与斑块稳定性的相关性  

Correlation Between Neovascularization in Atherosclerotic Plaques and Plaque Stability

作　　者：张一帆 李芳 李启玉 邵醒 ZHANG Yifan;LI Fang;LI Qiyu;SHAO Xing(Guizhou University of traditional Chinese Medicine,Guizhou Guiyang 550022,China;The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guizhou Guiyang 550002,China)

机构地区：[1]贵州中医药大学,贵州贵阳550022 [2]贵州中医药大学第二附属医院,贵州贵阳550002

出　　处：《中医药临床杂志》2024年第2期295-300,共6页Clinical Journal of Traditional Chinese Medicine

基　　金：贵州省2018年度科技支撑计划项目[2018]2771。

摘　　要：目的:探讨动脉粥样硬化内新生血管与斑块稳定性的相关性。方法:建立AS实验兔模型,将模型实验兔随机分为空白对照组、模型组、高脂饮食组、中药复方组、阿托伐他汀组。观察空白对照组、高脂饮食组第4周、8周、12周时血脂变化情况、用荧光定量聚合酶链式反应法检测组织标本中CD34的mRNA表达量、胸主动脉HE染色结果及新生血管内皮细胞标志物CD34水平的变化差异。结果:与空白组比较,高脂饮食组总胆固醇、甘油三酯、低密脂蛋白升高明显,高密度脂蛋白降低明显(P<0.05);与模型组比较,CD34的mRNA定量表达结果,高脂饮食组CD34的表达量>1,且增长明显(P<0.05);HE染色显示,高脂饮食组内膜下可见大量泡沫细胞形成纤维斑块病变;免疫组化显示,高脂饮食组有内膜改变,CD34染色呈阳性表达,且随时间的推移,阳性表达逐渐增强,内膜下可见大量蓝紫色细胞核聚集,为CD34标记的新生血管,高脂饮食组的实验兔斑块内新生血管在不断生长。结论:脂质代谢紊乱,低密脂蛋白、高密度脂蛋白水平与斑块内新生血管相关,新生血管为AS进展过程中重要的伴随现象,AS斑块不稳定性与斑块内新生血管密切相关,且呈正相关性。Objective:To explore the correlation between neovascularization and plaque stability in atherosclerosis(AS).Methods:An AS experimental rabbit model was established,and the model experimental rabbits were randomly divided into blank control group,model group,high-fat diet group,traditional Chinese medicine compound group,and atorvastatin group.Observe the changes in blood lipids in the blank control group and the high-fat diet group at 4,8,and 12 weeks,use PCR to detect CD34 mRNA expression in tissue specimens,thoracic aorta HE staining results,and neovascular endothelial cell marker CD34 Level changes.Results:Compared with the blank group,T-CHO,TG,and LDL-C in the high-fat diet group increased significantly,and HDL-C decreased significantly(P<0.05);compared with the model group,the quantitative expression results of CD34 mRNA in the high-fat diet group The expression of CD34 in the diet group was increased significantly(P<0.05);HE staining showed that in the high-fat diet group,a large number of foam cells could be seen under the intima to form fibrous plaque lesions;immunohistochemistry showed that in the high-fat diet group,there were endometrium The membrane changes,CD34 staining shows positive expression,and as time goes by,the positive expression gradually increases,and a large number of blue-purple cell nuclei can be seen under the intima,which are CD34-labeled new blood vessels.The new blood vessels in the plaques of experimental rabbits in the high-fat diet group Keep growing.Conclusion:Lipid metabolism disorder,LDL-C,HDL-C levels are related to new blood vessels in plaques.New blood vessels are an important accompanying phenomenon in the progression of AS.AS plaque instability is closely related to new blood vessels in plaques.And there is a positive correlation.

关 键 词：动脉硬化斑块 新生血管 CD34 

分 类 号：R-33[医药卫生]