miR-214/SOX4调控前列腺癌细胞的迁移和侵袭  

Migration and invasion of prostate cancer cells regulated by miR-214/SOX4

作　　者：徐广驰 刘涛 孟尹 王立鹤[1] 董波[1] 彭飞飞[1] 刘松涛 李淑奎 Xu Guangchi;Liu Tao;Meng Yin;Wang Lihe;Dong Bo;Peng Feifei;Liu Songtao;Li Shukui(Department of Urinary Surgery,the Second Affiliated Hospital of Qiqihar Medical University,Qiqihar,Heilongjiang 161000,China)

机构地区：[1]齐齐哈尔医学院附属第二医院泌尿外科,黑龙江齐齐哈尔161000

出　　处：《齐齐哈尔医学院学报》2023年第23期2221-2225,共5页Journal of Qiqihar Medical University

基　　金：齐齐哈尔市科学技术计划项目(SFGG-201932)。

摘　　要：目的探究miR-214是否通过靶向SOX4影响前列腺癌(PC)迁移与侵袭,为PC治疗提供潜在的治疗靶标。方法利用肿瘤基因组图谱数据库分析SOX4在癌及癌旁组织中的表达。用RT-qPCR和Western blotting方法检测了SOX4在前列腺癌细胞和正常前列腺细胞中的表达水平。用miR-214 mimics转染PC-3和DU145细胞,使miR-214过表达,并观察其对细胞功能的影响。结果PC组织和前列腺癌细胞系(PC3、DU145、22Rv1)中SOX4的表达水平上调,miR-214的表达下调。过表达的miR-214显著抑制癌细胞侵袭及迁移。结论miR-214通过负性调节SOX4的表达而发挥抑癌作用。SOX4可能是PC治疗的潜在靶点。Objective To investigate whether mir-214 affects the migration and invasion of prostate cancer(PC)by targeting SOX4,and provide potential therapeutic targets for the treatment of PC.Methods The expression of SOX4 in cancer and adjacent tissues was analyzed by tumor genome atlas database.The expression levels of SOX4 in prostate cancer cells and normal prostate cells were detected by RT-qPCR and Western blotting.PC-3 and DU145 cells were transfected with mir-214 mimics to make mir-214 over-express,and its effect on cell function was observed.Results The expression level of SOX4 was up-regulated and the expression of miR-214 was down-regulated in PC tissues and prostate cancer cell lines(PC3,DU145,22Rv1).Overexpression of miR-214 significantly inhibited the invasion and migration of cancer cells.Conclusions Mir-214 plays an inhibitory role in cancer by negatively regulating SOX4 expression.SOX4 may be a potential therapeutic target for PC.

关 键 词：前列腺癌 SOX4 miR-214 迁移 侵袭 

分 类 号：R737.25[医药卫生—肿瘤]