基于网络药理学和分子对接探究芪芝口服液提高家禽免疫功能的作用机制  

Exploring mechanism of action of Qizhi oral liquid improving immune function of poultry based on network pharmacology and molecular docking

作　　者：荣轩 房思远 朱永明 李育明 赵兵令 RONG Xuan;FANG Si-yuan;ZHU Yong-ming;LI Yu-ming;ZHAO Bing-ling

机构地区：[1]河北维尔利动物药业集团公司,河北石家庄050200 [2]河北省中兽药产业技术研究院,河北石家庄050200 [3]河北农业大学动物医学院,河北保定071000 [4]河北省功能性饲料添加剂技术创新中心,河北石家庄050200

出　　处：《饲料研究》2024年第2期70-76,共7页Feed Research

基　　金：河北省中兽药产业技术研究院建设补助经费(项目编号:225790267H);河北省功能性饲料添加剂技术创新中心建设补助经费(项目编号:217790537H)。

摘　　要：试验旨在探究芪芝口服液提高家禽免疫功能的作用机制。采用中药系统药理学数据库与分析平台(TCMSP)收集该复方中黄芪和灵芝2味药材的活性成分及其作用靶点,并与OMIM、TTD和GeneCards数据库收集免疫的靶点进行交集,获得芪芝口服液作用于家禽免疫的潜在靶点。运用Cytoscape 3.9.1软件构建中药-成分-靶点-通路-疾病网络图和蛋白质相互作用(PPI)网络,并对靶点进行GO富集和KEGG通路分析。使用分子对接技术进行活性成分和核心靶点的分子对接验证。结果显示,芪芝口服液30个成分对应66个靶点,家禽免疫329个靶点,其中交集靶点17个,PPI核心靶点包括核因子κB抑制因子α(NFKBIA)、白细胞介素-6(IL-6)和干扰素-γ(IFNG)等。GO功能富集共有38条结果,包括33个生物过程条目、4个分子功能条目和1个细胞成分条目;KEGG通路分析显示交集基因主要富集于C型凝集素受体信号通路、FoxO信号通路和Toll样受体等信号通路。分子对接结果显示,关键成分与核心蛋白靶点有较好的结合活性,证明了网络药理学预测结果的可靠性。研究表明,芪芝口服液可通过多成分、多靶点、多通路提升家禽的免疫功能。The aim of the experiment was to explore the mechanism of Qizhi oral liquid in enhancing the immune function of poultry.The active ingredients and their targets of Astragalus and Ganoderma lucidum in the compound were collected by the traditional Chinese medicine systematic pharmacology database and analysis platform(TCMSP),and the immune targets were collected by the OMIM,TTD and GeneCards databases.The potential target of Qizhi oral liquid for the poultry immunity was obtained.The Chinese medicine-component-target-pathway-disease network diagram and protein interaction(PPI)network were constructed by Cytoscape 3.9.1 software,and GO enrichment and KEGG pathway analysis were performed on the targets.The molecular docking of active ingredients and core targets was verified by molecular docking technology.The results showed that the 30 components of Qizhi oral liquid correspond to 66 targets,and 329 targets of poultry immunization,including 17 intersection targets.The core PPI targets included nuclear factorκB inhibitorα(NFKBIA),interleukin-6(IL-6)and interferonγ(IFNG).There were 38 results of GO functional enrichment,including 33 biological process items,four molecular function items and one cell component item.KEGG pathway analysis showed that the intersection genes were mainly concentrated in the C-type lectin receptor signaling pathway,the FoxO signaling pathway and the toll-like receptor signaling pathway.The results of molecular docking showed that the key components had good binding activity with the core protein target,which proved the reliability of the prediction results of network pharmacology.The study indicates that Qizhi oral liquid can enhance the immune function of poultry through a multi-component,multi-target and multi-pathway.

关 键 词：芪芝口服液 免疫功能 网络药理学 分子对接 家禽 

分 类 号：S816.7[农业科学—饲料科学]