褐藻寡糖的酶法制备及其在水产养殖中的应用  

Enzymatic preparation of alginate oligosaccharides and its application in aquaculture

作　　者：黄哲 胡睿同[1,2,3] 王连顺 丛玉婷 卢亚楠 王丽 王华 杨国军[1,2,3] HUANG Zhe;HU Rui-tong;WANG Lian-shun;CONG Yu-ting;LU Ya-nan;WANG Li;WANG Hua;YANG Guo-jun

机构地区：[1]大连海洋大学水产与生命学院,辽宁大连116023 [2]设施渔业教育部重点实验室(大连海洋大学),辽宁大连116023 [3]辽宁省贝类良种繁育工程技术研究中心,辽宁大连116023

出　　处：《饲料研究》2024年第2期139-145,共7页Feed Research

基　　金：辽宁省教育厅面上项目(项目编号:LJKZ0706,LJKMZ20221104);大连市青年科技之星项目(项目编号:2021RQ092);辽宁省科技厅博士科研启动基金计划(项目编号:2022-BS-278);大连海洋大学博士科研启动基金计划(项目编号:110222206);设施渔业教育部重点实验室(大连海洋大学)开放课题(项目编号:2021-MOEKLECA-KF-02202223);辽宁省海洋经济发展专项资金项目(项目编号:1638837263397)。

摘　　要：褐藻寡糖(AOS)是一类由2~10个单糖分子经相同或不同糖苷键连接而成的低分子量聚合物。褐藻胶裂解酶是酶法制备AOS的重要工具,酶法制备的AOS在纯度、性能、结构方面优于其他方法制备的AOS。文章综述了褐藻胶裂解酶的来源、结构及酶学性质,总结了酶法制备的AOS在饲料添加剂、微藻培育、免疫调节、抗氧化和抗菌等水产养殖方面的应用,对褐藻胶裂解酶的研究方向以及AOS在水产养殖中的应用前景进行了展望,为褐藻胶裂解酶、AOS在水产养殖中的应用提供参考。Alginate oligosaccharides are a kind of low molecular weight polymer composed of 2~10 monosaccharide molecules linked by the same or different glycosidic bonds.Alginate lyase is an important tool for the enzymatic preparation of AOS.AOS prepared by the enzymatic method is superior to AOS prepared by other methods in purity,performance and structure.In this paper,the origin,structure and enzymatic properties of alginate lyase were reviewed,and the applications of alginate lyase in aquaculture,such as feed additive,microalgae cultivation,immune regulation,antioxidant and antibacterial,were summarized.The research direction of alginate lyase and the application prospect of alginate lyase in aquaculture were prospected,which provided reference for the application of alginate lyase and AOS in aquaculture.

关 键 词：褐藻寡糖 褐藻胶裂解酶 酶法制备 水产养殖 生物活性 免疫调节 

分 类 号：S963[农业科学—水产养殖]