PRiME HLB前处理技术联合超高效液相色谱-三重四极杆质谱系统检测水产品中17种磺胺类药物残留  

PRiME HLB Pretreatment Technology Combined with Ultra-High Performance Liquid Chromatography-Triple Quad Mass Spectrometry System for the Detection of 17 Sulfonamide Residues in Aquatic Products

作　　者：余海芬[1] 徐桦[1] 吴真善 包蔚[1] 朱晓芸[1] 何锴[1] 黄忠[1] 战涛[1] YU Haifen;XU Hua;WU Zhenshan;BAO Wei;ZHU Xiaoyun;HE Kai;HUANG Zhong;ZHAN Tao(Jiading District of Shanghai Agricultural Techonogy Extension Service Center,Shanghai 201800,China)

机构地区：[1]上海市嘉定区农业技术推广服务中心,上海201800

出　　处：《现代食品》2024年第1期213-215,224,共4页Modern Food

摘　　要：使用通过型固相萃取小柱PRi ME HLB处理水产品样品,建立了一种水产品中17种磺胺类药物的简单、快速的筛选分析方法。水产品样品经80%乙腈水溶液(含0.2%甲酸)提取,过PRi ME HLB固相萃取柱净化,浓缩后经C_(18)色谱柱梯度洗脱分离,超高效液相色谱-三重四极杆质谱系统进行定量分析。结果表明,17种磺胺类药物在1.0~50.0 ng·mL^(-1)线性关系良好,相关系数R^(2)>0.99;该方法检出限为2μg·kg^(-1);添加浓度为10μg·kg^(-1)时方法回收率在71.3%~118.4%,RSD值均小于20%。A simple and rapid screening and analysis method for 17 sulfonamide drugs in aquatic products was established by using a PRiME HLB solid phase extraction column to treat aquatic product samples.Aquatic product samples were extracted with an 80%acetonitrile aqueous solution(containing 0.2%formic acid),purified by PRiME HLB solidphase extraction column,concentrated and separated by C_(18) chromatographic column gradient elution.Quantitative analysis was performed using ultra high performance liquid chromatography triple quadrupole mass spectrometry system.The results showed that the 17 sulfonamide drugs in this method exhibit a good linear relationship within the concentration range of 1.0~50.0 ng·mL^(-1),with correlation coefficients(R^(2))greater than 0.99;the method detection limit is 2μg·kg^(-1);when the concentration of 10μg·kg^(-1)was added,the recovery rate of the method ranged from 71.3%to 118.4%,and the RSD values were all less than 20%.

关 键 词：磺胺 水产品 PRiME HLB固相萃取小柱 

分 类 号：O657.63[理学—分析化学] TS254.7[理学—化学]