血型CD59基因在胰腺癌中的表达及临床意义  

Expression and Clinical Significance of Blood Group CD59 Gene in Pancreatic Cancer

作　　者：周仁龙 黄国清 李凌波 唐丹丹 ZHOU Renlong;HUANG Guoqing;LI Lingbo;TANG Dandan(Department of Blood Transfusion,Longhua District Central Hospital,Shenzhen,Guangdong 518110;Aikang MedTech Co.,Ltd;Headquarters of Nanshan Medical Group,Shenzhen,Guangdong 518055)

机构地区：[1]深圳市龙华区中心医院输血科,广东518110 [2]吉林省健康管理学会、深圳爱康生物科技股份有限公司 [3]深圳市南山区医疗集团总部,广东518055

出　　处：《临床输血与检验》2024年第1期101-109,共9页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的运用生物信息学技术分析CD59血型基因在胰腺癌(pancreatic adenocarcinoma,PAAD)中的表达水平以及临床意义。方法通过GEPIA、UALCAN和HPA数据库分析PAAD中CD59的mRNA和蛋白表达水平。通过TIMER数据库分析CD 59在PAAD中与免疫细胞浸润的相关性。通过UALCAN数据库分析CD59参与信号通路影响PAAD的进展。通过STRING数据库分析CD59表达与上下游蛋白的相互作用。基于单因素Cox,多因素Cox回归结果分析CD59血型与PAAD预后相关。结果与癌旁组织相比,CD59在PAAD中的mRNA和蛋白表达水平显著升高(P<0.05),与CD59低表达PAAD患者相比,高表达CD59的患者预后差。CD59在PAAD中表达水平与CD8+T细胞,巨噬细胞、中性粒细胞、树突状细胞呈正相关(P<0.05)。基于单因素Cox和多因素Cox回归分析高表达CD 59是PAAD预后危险因素。KEGG富集分析占主导地位的是补体和凝血级联通路,GO富集分析占主导地位的是蛋白质加工的调控信号通路。结论CD59血型基因在PAAD中呈现高表达,与PAAD的发生、发展和预后不良相关,有望作为PAAD诊断、预后判断和治疗靶点。Objective To investigate the expression levels and clinical significance of the CD59 blood type gene in pancreatic adenocarcinoma(PAAD)using bioinformatics techniques.Methods CD59 mRNA and protein expression in PAAD were analyzed via GEPIA,UALCAN,and HPA databases.The TIMER database was utilized to examine the correlation between CD59 in PAAD and immune cell infiltration,UALCAN database to investigate how CD59 in PAAD affected signaling pathways influencing PAAD progression,STRING database to explore the interaction between CD59 expression and upstream/downstream proteins,and Single-factor Cox and multi-factor Cox regression results to assess the association between CD59 blood type and PAAD prognosis.Results mRNA and protein expression levels of CD59 were significantly elevated in PAAD compared to adjacent tissues(P<0.05).Patients with high CD59 expression had a poorer prognosis than those with low CD59 expression in PAAD.The expression of CD59 in PAAD correlated positively with CD8+T cells,macrophages,neutrophils,and dendritic cells(correlation coefficient>0,P<0.05).Single-factor and multi-factor Cox regression analyses established high CD59 expression as a risk factor for PAAD prognosis.KEGG enrichment analysis revealed a predominant role of the complement and coagulation cascades,while GO enrichment analysis indicated the dominance of regulatory signaling pathways in protein processing.Conclusion The CD59 blood type gene exhibits higher expression in PAAD and correlation with its occurrence,development,and adverse prognosis,suggesting its potential as a diagnostic/prognostic indicator and therapeutic target for PAAD.

关 键 词：胰腺癌 生物信息学 CD59基因 数据库 

分 类 号：R735.9[医药卫生—肿瘤]