原生质体形成及融合因素分析  

Analysis of Influencing Factors of Protoplast Formation

作　　者：常玉广 金露 CHANG Yu-guang;JIN Lu(School of Environmental Science,Nanjing Xiaozhuang University,Nanjing 211171,China)

机构地区：[1]南京晓庄学院环境科学学院,江苏南京211171

出　　处：《南京晓庄学院学报》2023年第6期53-56,共4页Journal of Nanjing Xiaozhuang University

基　　金：南京晓庄学院大学生科研基金(2023XSKY047).

摘　　要：本实验以经过青霉素筛选后的两株絮凝菌为对象,分别测定其溶菌酶处理前总菌数、溶菌酶处理后总菌数和原生质体再生菌数,计算原生质体形成率和再生率,并对PEG的作用浓度和时间进行优化。实验结果:F2原生质体形成率为99.97%,F6原生质体形成率为99.96%,F2原生质体再生率为0.4225%,F6原生质体再生率为0.5423%,初步判断溶菌酶处理后得到的原生质体在质量和数量上满足细胞融合需求,确定了原生质体融合PEG最佳作用浓度为30%,最佳作用时间为10 min,以保证原生质体融合的完成。Two strains of flocculating bacteria screened by penicillin were studied in this experiment.The total number of bacteria before and after lysozyme treatment,and the number of protoplast regeneration were measured respectively.The protoplast formation rate and regeneration rate were calculated,and the effect concentration and time of PEG were optimized.The results showed that the protoplast formation rate of F2 was 99.97%,the protoplast formation rate of F6 was 99.96%,the protoplast regeneration rate of F2 was 0.4225%,and the protoplast regeneration rate of F6 was 0.5423%.Thus,the protoplasts obtained after lysozyme treatment can meet the requirements of cell fusion in quality and quantity.The optimal concentration of PEG was 30%and the optimal time of PEG was 10min in protoplast fusion,ensuring the successful completion of the protoplast fusion process.

关 键 词：原生质体 再生 融合 PEG 

分 类 号：X172[环境科学与工程—环境科学]