CGRP对离体脊髓运动神经元突触传递的影响  

Effects of CGRP on synaptic transmissions in spinal cord motoneuron in vitro

作　　者：杜幼梅 周方云 汪萌芽[1] DU Youmei;ZHOU Fangyun;WANG Mengya(Cell Electrophysiology Laboratory,Wannan Medical College,Wuhu 241002,Anhui,China)

机构地区：[1]皖南医学院细胞电生理研究室,安徽芜湖241002

出　　处：《皖南医学院学报》2024年第1期1-5,共5页Journal of Wannan Medical College

基　　金：国家自然科学基金项目(31271155)。

摘　　要：目的:观察降钙素基因相关肽(CGRP)对新生大鼠离体脊髓运动神经元(MN)突触传递的影响。方法:选取8~12日龄的新生SD大鼠制备离体脊髓横切片,运用细胞内记录技术进行MN生物电记录,在同侧腹外侧索(iVLF)和同侧背根(iDR)施加电刺激(单脉冲,0.1~0.2 ms,0.1 Hz,10~100 V)以诱发兴奋性突触后电位(EPSP),即iVLF-EPSP和iDR-EPSP。给予CGRP灌流,观察其对脊髓MN突触传递的影响。结果:①在7个稳定记录的MN,诱导出iVLF-EPSP并灌流1μmol/L CGRP 15 min,在其中的5个MN上观察到iVLF-EPSP的时程被缩短(P<0.05)。②在5个稳定记录的MN,诱导出iDR-EPSP并灌流1μmol/L CGRP 15 min,在其中的3个MN上观察到iDR-EPSP的曲线下面积被抑制(P<0.05)、上升时间被缩短(P<0.01)。③在2个稳定记录的MN中,诱导出iDR-EPSP并灌流1、5μmol/L CGRP各15 min,观察到iDR-EPSP幅度的平均抑制率分别为6%、36%。结论:CGRP对部分脊髓MN的下行激活和外周传入突触传递可能存在抑制作用。Objective:To in vitro observe the effects of calcitonin gene-related peptide(CGRP)on synaptic transmissions in the spinal cord motoneuron(MN)of neonatal rat.Methods:Neonatal SD rats,aged 8 to 12 days,were selected to prepare transverse spinal cord slices.Intracellular recording was performed from MN in the ventral horn of the spinal cord slices,and stimulation(monopulse,0.1 to 0.2 ms,0.1 Hz,10 to 100 V)was applied to the ipsilateral ventrolateral funiculus(iVLF)and ipsilateral dorsal root(iDR)to induce excitatory postsynaptic potentials(iVLF-EPSP and iDR-EPSP).CGRP was perfused to observe its effects on the synaptic transmissions in spinal cord MN.Results:①In 7 stably recorded MN,iVLF-EPSP was evoked and 1μmol/L CGRP was perfused for 15 min.Inhibitory effect on duration of iVLF-EPSP was observed in 5 MN(P<0.05);②In 5 stably recorded MN,iDR-EPSP was evoked and 1μmol/L CGRP was perfused for 15 min.Inhibitory effect on area under curve(P<0.05)and raise time(P<0.01)of iDR-EPSP was observed in 3 MN;③In 2 stably recorded MN,the amplitude of iDR-EPSP was decreased after perfusion with 1 and 5μmol/L CGRP respectively for 15 min,with the inhibition rates of 6%and 36%,respectively.Conclusion:CGRP may produce inhibitory effects on MN synaptic transmissions from descending activation and sensory afferent input pathways.

关 键 词：降钙素基因相关肽 运动神经元 突触传递 脊髓 

分 类 号：R-332[医药卫生] R338.1R744.8