YTHDC1介导的CENPK调节人宫颈癌细胞侵袭和增殖  

YTHDC1 mediated CENPK regulating human cervical cancer cell invasion and proliferation

作　　者：顾艳 赵夏丽 王梅 倪观太 GU Yan;ZHAO Xiali;WANG Mei;NI Guantai(Department of Obstetrics and Gynecology,The First Affiliated Hospital of Wannan Medical College,Wuhu 241001,Anhui,China)

机构地区：[1]皖南医学院第一附属医院弋矶山医院妇产科,安徽芜湖241001

出　　处：《皖南医学院学报》2024年第1期19-23,共5页Journal of Wannan Medical College

基　　金：安徽高校研究生科学研究项目(YJS20210545)。

摘　　要：目的:找寻宫颈癌的预测、治疗靶点,探究靶点与N6-甲基腺苷(m6A)核酸修饰之间的联系及发挥的生物学功能。方法:在GEPIA2数据库提供的数据中筛选宫颈癌差异表达的基因。EdU实验、划痕实验以及Transwell实验分别检测着丝粒蛋白K(CENPK)对宫颈癌细胞增殖、迁移和侵袭能力的影响。RNA m6A修饰的定量测定结合蛋白质印迹法研究m6A修饰与CENPK高表达的关系。结果:CENPK在宫颈癌组织中上调(P<0.05)。沉默CENPK基因导致宫颈癌细胞增殖能力下降,抑制了细胞迁移和侵袭(P<0.01)。m6A阅读器YTH域包含蛋白1(YTHDC1)通过降低CENPK蛋白的表达在宫颈癌细胞中发挥作用(P<0.01)。结论:CENPK可能是一个潜在的宫颈癌治疗靶点。Objective:To identify the prediction and treatment targets of cervical cancer,and investigate the relationship between these targets and N6-methyladenosine(m6A)nucleic acid modification and their respective biological functions.Methods:GEPIA2 database was used to screen differentially expressed genes in cervical cancer.EdU assay,scratch assay and Transwell assay were used to detect the effects of CENPK on the proliferation,migration,and invasion of cervical cancer cells.The relationship between m6A modification and high expression of CENPK was studied by combining quantitative determination of RNA m6A modification with Western blot.Results:CENPK expression was upregulated in cervical cancer tissues(P<0.05).Silencing the CENPK gene resulted in decreased proliferation,migration and invasion of cervical cancer cells(P<0.01).The m6A reader protein YTHDC1 was involved in cervical cancer by reducing the expression of CENPK protein(P<0.01).Conclusion:These results suggest that CENPK is a potential therapeutic target in cervical cancer.

关 键 词：宫颈癌 着丝粒蛋白K YTH域包含蛋白1 肿瘤细胞 侵袭 增殖 

分 类 号：R737.33[医药卫生—肿瘤] R329.28[医药卫生—临床医学]